【促销内容】
即日起到6月28日,凡订购Pull-down & G-LISA® Kits产品即可享受第二件6折。产品清单请联系优宁维索取。
【About Activation Assays】
Since 2001, Cytoskeleton has provided the scientific communitywith the most robust, accurate, and time-saving kits to measureSmall GTP-binding protein (SmG) activation. Along the way, wehave developed numerous versions for different SmGs, suchas Rho, Rac, Arf1 & 6, Ras, and Ral. Also, the quantifiable GLISAversions enabled a new wave of more sensitive applications, e.g.measurement in limited primary cell numbers and Matrigel 3Dmatrix. We continue to develop and maintain these high standards,which allow you to produce the best results in the least amount of time.
【Comparison of Pulldown and GLISA formats】
Parameter |
Pull-down |
G-LISA® |
Total protein per assay |
500-2000 μg |
10-50 μg |
Assay time |
10-12 h (2 days) |
<3 h |
Primary cells & 3D matrix compatible |
No |
Yes |
Sample handling |
10 Samples |
96 Samples |
Quantitative data* |
Semi |
Yes |
* Numerical readouts and fewer sample handling steps make G-LISA® assays more quantitative. |
Pull-down Result Example |
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G-LISA® Result Example |
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Swiss 3T3 cells were serum-starved for 24h; after this, a sample was treated with 10 ng/ml of EGF for 2 min (Lanes4 & 5). Other cells were not treated and remained serumstarved (Lanes 2 & 3). Rac1 activation was measured using the Rac1 Activation pull-down assay. 500 μg of lysate were assayed with 10 μg of PAK-PBD beads (Lanes 2-5). Lane 1 shows 20 ng of recombinant Rac1-His protein run as a Western blot standard. |
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Time course of activation of RhoA in Swiss 3T3 cells by CN01 and LPA. Serum-starved Swiss 3T3 cells were treated with Rho Activator I, Cat. # CN01 (blue diamonds) or LPA (red squares). RhoA activity was measured by reading signals at OD490nm. Data are background subtracted. |
【Pull-down Activation Assays】
Pull-down assays utilize affinity beads linked to aneffector protein that selectively binds active GTPasefollowed by quantitation with Western blotting.
Pull-down Activation Assays |
Cat. # |
Amount |
Combo RhoA/Rac1/Cdc42 Activation Assay Biochem Kit™ |
BK030 |
3 x 10 assays |
Arf1 Activation Assay Biochem Kit™ |
BK032-S |
20 assays |
Arf6 Activation Assay Biochem Kit™ |
BK033-S |
20 assays |
Cdc42 Activation Assay Biochem Kit™ |
BK034-S
BK034 |
20 assays
50 assays |
Rac1 Activation Assay Biochem Kit™ |
BK035-S
BK035 |
20 assays
50 assays |
RalA Activation Assay Biochem Kit™ |
BK040 |
50 assays |
Ras Activation Assay Biochem Kit™ |
BK008-S
BK008 |
20 assays
50 assays |
RhoA Activation Assay Biochem Kit™ |
BK036-S
BK036 |
20 assays
80 assays |
Protease Inhibitor Cocktail (100x stock) |
PIC02 |
1 ml |
【 G-LISA® Pull-down Activation Assays Activation Assays 】
G-LISAs use a 96-well plate coated with effector protein that selectively binds the active GTPase followed by quantitation with ELISA techniques.
G-LISA Activation Assays |
Cat. # |
Amount |
RhoA/Rac1/Cdc42 G-LISA Activation Assay Bundle
BK135=BK124-S+BK127-S+BK128-S |
BK135 |
3 Kits
(24 assays/kit) |
Arf1 G-LISA® Activation Assay, colorimetric |
BK132 |
96 assays |
Arf6 G-LISA® Activation Assay, colorimetric |
BK133 |
96 assays |
Cdc42 G-LISA® Activation Assay, colorimetric |
BK127-S
BK127 |
24 assays
96 assays |
Rac1,2,3 G-LISA® Activation Assay, colorimetric |
BK125 |
96 assays |
Rac1 G-LISA® Activation Assay, colorimetric |
BK128-S
BK128 |
24 assays
96 assays |
Rac1 G-LISA® Activation Assay, luminescence |
BK126 |
96 assays |
RalA G-LISA® Activation Assay, colorimetric |
BK129 |
96 assays |
Ras G-LISA® Activation Assay, colorimetric |
BK131 |
96 assays |
RhoA G-LISA® Activation Assay, colorimetric |
BK124-S
BK124 |
24 assays
96 assays |
RhoA G-LISA® Activation Assay, luminescence |
BK121 |
96 assays |
Protease Inhibitor Cocktail (100x stock) |
PIC02 |
1 ml |
上海优宁维生物科技股份有限公司
您可以通过以下方式了解更多:
热线:4008-168-068 邮箱:info@univ-bio.com 微信:优宁维抗体专家
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