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rhFABP3, CF (100 UG)
rhFABP3, CF (100 UG)

rhFABP3, CF (100 UG)

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    产品介绍
    产品信息

    Carrier Free

    What does CF mean?

    CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

    What formulation is right for me?

    In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

    9479-CL

    FormulationLyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT.
    ReconstitutionReconstitute at 1 mg/mL in PBS.
    ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

    Recombinant Human FABP3/H-FABP Protein, CF Summary

    Product Specifications

    Purity
    >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
    Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
    Activity
    Bioassay data are not available.
    Source
    E. coli-derived human FABP3/H-FABP protein
    MHHHHHHGS Human FABP3/H-FABP
    (Met1-Ala133)
    Accession # P05413
    N-terminusC-terminus
    Accession #
    P05413
    N-terminal Sequence
    Analysis
    Met
    Predicted Molecular Mass
    16 kDa
    SDS-PAGE
    15 kDa, reducing conditions
    背景
    别名
    FABP11,FABP3,fatty acid binding protein 11,fatty acid binding protein 3, muscle and heart (mammary-derived growthinhibitor),Fatty acid-binding protein 3,Fatty acid-binding protein 3, muscle,fatty acid-binding protein, heart,Heart-type fatty acid-binding protein,HFABP,H-FABP,H-FABPM-FABP,Mammary-derived growth inhibitor,MDGI,Muscle fatty acid-binding protein,O-FABP,Fatty Acid-Binding Protein 3
    背景

    Background: FABP3/H-FABP

    Fatty acid binding protein-3 (FABP3; also named Fatty acid binding protein 11, FABP11, H- or M- (heart or muscle) FABP, Mammary derived growth inhibitor, or MDGI) is a member of a large superfamily of lipid binding proteins that are expressed in a tissue specific manner (1, 9, 10). FABP3 is one of ten cytoplasmic FABPs that are 14-15 kDa in size and range from 126-140 amino acids (aa) in length (1, 2, 3). Although all are highly conserved in their tertiary structure, there is only modest aa identity between any two members. The FABP family members are subdivided based on organ or tissue type it was originally expressed or identified; liver- (L-FABP), intestine- (I-FABP), heart- (H-FABP), adipocyte- (A-FABP), epidermal- (E-FABP), ileal- (IL-FABP), brain- (B-FABP), myelin- (M-FABP) and testis-FABP (T-FABP) (1). Human H-FABP, the product of the FABP3 gene, is a 132 aa cytosolic protein that shows a flattened beta -barrel structure generated by a series of antiparallel beta ‑strands and two alpha ‑helices (4, 5, 7). One molecule of FABP3 is capable of binding one long-chain fatty acid (6, 8, 11). It is suggested that ligands first bind to the outside of the molecule, and this binding subsequently induces a conformational change in the binding protein, resulting in "internalization" of the ligand (7, 11). Human FABP3 is 86%, 89% and 89% aa identical to mouse, rat and canine FABP3, respectively. It also shows 29% and 32% aa identity to human L-FABP and I‑FABP, respectively.

    References
    1. Smathers, R & Petersen, D. (2011) Human Genomics 5:170.
    2. Storch, J. & Thumser, AE. (2000) Biochim Biophys Acta. 1486:28.
    3. Mihajlovic, M. & Lazaridis, T. (2007) Protein Sci. 9:2042.
    4. Sweetser, D.A. et al. (1987) J. Biol. Chem. 262:16060.
    5. Offner, G. et al. Biochem. J. (1988) 252:191.
    6. Glatz JF. et al. (1985) Chem Phys Lipids. 38:115.
    7. Bernlohr, DA. et al. (1997) Ann. Rev. of Nut. 17:277.
    8. Zanotti G. et al. (1992) J. Biol. Chem. 267:18541.
    9. Zimmerman, A.W. & J.H. Veerkamp (2002) Cell. Mol. Life Sci. 59:1096.
    10. Haunerland, N.H. and F. Spener (2004) Prog. Lipid Res. 43:328.
    11. Majava,V. et al. (2010) PLoS One. 5:e10300.
    Long Name
    Fatty Acid-Binding Protein 3
    Entrez Gene IDs
    2170 (Human)
    Alternate Names
    FABP11; FABP3; fatty acid binding protein 11; fatty acid binding protein 3, muscle and heart (mammary-derived growthinhibitor); Fatty acid-binding protein 3; Fatty acid-binding protein 3, muscle; fatty acid-binding protein, heart; Heart-type fatty acid-binding protein; HFABP; H-FABP; H-FABPM-FABP; Mammary-derived growth inhibitor; MDGI; Muscle fatty acid-binding protein; O-FABP
    研究领域
    制备和贮存
    溶解方法
    Reconstitute at 1 mg/mL in PBS.

    参考图片

    R&D Systems proteins are almost always sold with a bioassay to indicate activity. However, we recognize that sometimes proteins might be novel, and their bioactivity may not be well understood. In addition, some researchers may wish to use polypeptides to make antibodies. To facilitate the advancement of new science, we now offer our Innovator Series of proteins.

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    货号：
    9479-CL-100
    一键复制
    询价
    100UG
    选择数量
    当前规格1件起购 
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