hCD133 APC MAb (Cl 1 (100 Tests)

hCD133 APC MAb (Cl 1 (100 Tests)

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品牌: R&D Systems
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    来源宿主:
    Mouse IgG2a
    Mouse IgG2a
    实验应用:
    Flow Cytometry
    Flow Cytometry
    产品介绍
    产品信息
    耦联标记
    APC
    纯化方式
    Protein A or G purified from hybridoma culture supernatant
    抗原名称
    AC133 Stargardt disease 4 (autosomal dominant), AC133, CD133 retinal 2, CD133, PROM1, prominin (mous
    宿主
    Mouse IgG2a
    免疫原
    NS0 mouse myeloma cell line transfected with human CD133
    Accession # O43490
    克隆号
    170411

    Scientific Data

    Flow Cytometry Detection of CD133 antibody in NS0 Mouse Cell line Transfected with Human CD133 antibody by Flow Cytometry.View Larger

    Detection of CD133 in NS0 Mouse Cell line Transfected with Human CD133 by Flow Cytometry. NS0 mouse myeloma cell line transfected with either human CD133 (filled histogram) or irrelevant transfectants (open histogram) was stained with Mouse Anti-Human CD133 APC‑conjugated Monoclonal Antibody (Catalog # FAB11331A). View our protocol for Staining Membrane-associated Proteins.

    Human CD133 APC-conjugated Antibody Summary

    Species Reactivity
    Human
    Specificity
    Detects human CD133.  Stains human CD133 transfectants but not irrelevant transfectants in flow cytometry.
    Source
    Monoclonal Mouse IgG2A Clone # 170411
    Purification
    Protein A or G purified from hybridoma culture supernatant
    Immunogen
    NS0 mouse myeloma cell line transfected with human CD133
    Accession # O43490
    Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
    Label
    Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

    Applications

    Recommended Concentration
    Sample
    Flow Cytometry
    10 µL/106 cells
    See below

    Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

    应用
    实验应用
    Flow Cytometry
    背景
    别名
    AC133 Stargardt disease 4 (autosomal dominant),AC133,CD133 retinal 2,CD133,PROM1,prominin (mouse)-like 1,Prominin 1,PROML1
    背景

    Background: CD133

    CD133 antigen also known as prominin-1 is a glycoprotein that in humans is encoded by the PROM1 gene. It is a pentaspan transmembrane glycoprotein, with highly restricted expression on plasma membrane protrusions of epithelial and other cell types.  CD133 is expressed in hematopoietic stem cells, endothelial progenitor cells, glioblastoma, neuronal and glial stem cells, as well as adult kidney, mammary glands, trachea, salivary glands, placenta, digestive tract, and testes. CD133 is used as a main marker for hematopoietic stem cells in transplant models. It has also been described as a marker of tumor initiating cells. 

    Entrez Gene IDs
    8842 (Human); 19126 (Mouse)
    Alternate Names
    AC133 Stargardt disease 4 (autosomal dominant); AC133; CD133 retinal 2; CD133; PROM1; prominin (mouse)-like 1; Prominin 1; PROML1
    制备和贮存
    保存方式

    Preparation and Storage

    Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.

    参考图片

    Detection of CD133 in NS0 Mouse Cell line Transfected with Human CD133 by Flow Cytometry. NS0 mouse myeloma cell line transfected with either human CD133 (filled histogram) or irrelevant transfectants (open histogram) was stained with Mouse Anti-Human CD133 APC‑conjugated Monoclonal Antibody (Catalog # FAB11331A). View our protocol for Staining Membrane-associated Proteins.

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    货号:
    FAB11331A-100
    一键复制
    询价
    25Tests
    100Tests
    选择数量
    当前规格1件起购 
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