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hADORA2A A647 MAb (C (100 Tests)

品牌： R&D Systems

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来源宿主：

Mouse IgG2a

实验应用：

Flow Cytometry

产品介绍

产品信息

耦联标记

Alexa Fluor 647

纯化方式

Protein A or G purified from hybridoma culture supernatant

抗原名称

A2aR， adenosine A2 receptor， Adenosine A2a R， adenosine A2a receptor， Adenosine A2aR， adenosine rece

宿主

Mouse IgG2a

免疫原

NS0 mouse myeloma cell line transfected with human Adenosine A2a R
Met1-Ser412
Accession # P29274

克隆号

599717

Scientific Data

Flow Cytometry

View Larger

Detection of Adenosine A2a R in HEK293 Human Cell Line Transfected with Human Adenosine A2a R and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human Adenosine A2a R or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Adora A2 R Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB94971R). Quadrant markers were set based on control antibody staining (Catalog # IC003R). View our protocol for Staining Membrane-associated Proteins.

Human Adenosine A2aR Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Adenosine A2a R in HEK293 human cell line transfected with Human Adenosine A2a R and not irrelavant transfectants in flow cytometry.

Source

Monoclonal Mouse IgG_2A Clone # 599717

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human Adenosine A2a R
Met1-Ser412
Accession # P29274

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

5 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

应用

实验应用

Flow Cytometry

背景

别名

A2aR,adenosine A2 receptor,Adenosine A2a R,adenosine A2a receptor,Adenosine A2aR,adenosine receptor A2a,adenosine receptor subtype A2a,ADORA 2,ADORA2,ADORA2A,hA2aR,RDC8,Adenosine A2A Receptor

背景

Background: Adenosine A2aR

ADORA-A2 (Adenosine A2a Receptor) is a widely expressed seven transmembrane G protein-coupled receptor. Activation by adenosine leads to increased intracellular cAMP levels. ADORA-2A mediates many biological functions, including cardiac rhythm and circulation, cerebral and renal blood flow, immune function, pain regulation, and sleep. ADORA-A2 has been identified as a target for therapeutic drugs for inflammation, cancer, ischemic reperfusion injury, diabetic nephropathy, infectious diseases and neuronal disorders.

Long Name

Adenosine A2A Receptor

Entrez Gene IDs

135 (Human); 11540 (Mouse); 25369 (Rat)

Alternate Names

A2aR; adenosine A2 receptor; Adenosine A2a R; adenosine A2a receptor; Adenosine A2aR; adenosine receptor A2a; adenosine receptor subtype A2a; ADORA 2; ADORA2; ADORA2A; hA2aR; RDC8

研究领域

Related Research Areas

制备和贮存

保存方式

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

参考图片

Detection of Adenosine A2a R in HEK293 Human Cell Line Transfected with Human Adenosine A2a R and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human Adenosine A2a R or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Adora A2 R Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB94971R). Quadrant markers were set based on control antibody staining (Catalog # IC003R). View our protocol for Staining Membrane-associated Proteins.

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货号：

FAB94971R-100

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询价

100Tests

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