Calcein

A fluorescent calcium probe

Calcein is a xanthene that is commonly used for the fluorometric determination of calcium in solution.1,2 As this form of calcein is not membrane permeable, it can be used in assays that evaluate membrane integrity.3,4 Calcein can also be used as a fluorescent indicator for fluoride, iron, and mercury.5,6,7 Excitation and emission maxima for calcein are 494 and 517 nm, respectively.2

622.5

Fluorescein Complexone|NSC 298193

OC1=CC2=C(C=C1CN(CC(O)=O)CC(O)=O)C3(C(C=CC=C4)=C4C(O3)=O)C5=CC(CN(CC(O)=O)CC(O)=O)=C(O)C=C5O2

C30H26N2O13

1461-15-0

≥ 4 years

1 M NaOH: 50 mg/ml PBS (pH 7.2): 0.2 mg/ml

1.Bandrowski, J.F. and Benson, C.L. Investigation of the use of calcein in the ultramicro fluorometric determination of calcium in serum. Clinical Chemistry 18(11), 1411-1414 (1972).
2.Sabnis, R.W. Handbook of biological dyes and stains: Synthesis and industrial applications. (2010).
3.Cutler, A.J. and Saleem, M. Permeabilizing soybean protoplasts to macromolecules using electroporation and hypotonic shock. Plant Physiology 83(1), 24-28 (1987).
4.Kendall, D.A. and MacDonald, R.C. A fluorescence assay to monitor vesicle fusion and lysis. The Journal of Biological Chemisty 257(23), 13892-13895 (1982).
5.Li, H.B. and Chen, F. A highly sensitive fluorimetric method for the determination of fluoride in biological material with Al3+-calcein complex. Fresenius.J.Anal.Chem. 368(5), 501-504 (2000).
6.Ali, A.,Zhang, Q.,Dai, J., et al. Calcein as a fluorescent iron chemosensor for the determination of low molecular weight iron in biological fluids. BioMetals 16(2), 285-293 (2003).
7.Lista, A.G.,Palomeque, M.E. and Fernández Band, B.S. Flow-injection fluorimetric determination of mercury(II) with calcein. Talanta 50(4), 881-885 (1999).