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HNE MAb (Cl 198960) (100 ug)
HNE MAb (Cl 198960) (100 ug)
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HNE MAb (Cl 198960) (100 ug)

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    来源宿主：
    Mouse IgG2b
    Mouse IgG2b
    实验应用：
    Western Blot(1 µg/mL)
    Simple Western(5 µg/mL)
    Western Blot(1 µg/mL)
    Simple Western(5 µg/mL)
    产品介绍
    产品信息
    耦联标记
    Unconjugated
    宿主
    Mouse IgG2b
    克隆号
    198960

    Scientific Data

    Western Blot Detection of Human 4-Hydroxynonenal antibody by Western Blot.View Larger

    Detection of Human 4‑Hydroxynonenal by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 4-Hydroxynonenal (4-HNE). PVDF membrane was probed with 1 µg/mL of Mouse Anti-4-Hydroxynonenal Monoclonal Antibody (Catalog # MAB3249) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for 4-Hydroxynonenal at adducts of histidine residues (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

    Simple Western Detection of Human 4-Hydroxynonenal antibody by Simple Western<SUP>TM</SUP>.View Larger

    Detection of Human 4‑Hydroxynonenal by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 4-Hydroxynonenal (4-HNE), loaded at 0.2 mg/mL. Specific bands were detected for 4‑Hydroxynonenal at adducts of histidine residues kDa (as indicated) using 5 µg/mL of Mouse Anti-4‑Hydroxynonenal Monoclonal Antibody (Catalog # MAB3249). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

    Immunohistochemistry 4-Hydroxynonenal antibody in Human Prostate by Immunohistochemistry (IHC-P).View Larger

    4‑Hydroxynonenal in Human Prostate. 4-Hydroxynonenal was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-4-Hydroxynonenal Monoclonal Antibody (Catalog # MAB3249) at 0.1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

    Immunohistochemistry 4-Hydroxynonenal antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P).View Larger

    4‑Hydroxynonenal in Human Prostate Cancer Tissue. 4-Hydroxynonenal was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-4-Hydroxynonenal Monoclonal Antibody (Catalog # MAB3249) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

    Immunocytochemistry/ Immunofluorescence Detection of Mouse 4-Hydroxynonenal by Immunocytochemistry/ImmunofluorescenceView Larger

    Detection of Mouse 4-Hydroxynonenal by Immunocytochemistry/Immunofluorescence Diapocynin inhibits the formation of 3-nitrotyrosine (3-NT) and 4-hydroxynonenal (4-HNE) in the substantia nigra (SN) of MPTP-treated mice. (A) Representative Western blots illustrating the expression of 3-NT in SN. (B) Bar graph showing mean Western blot 3-NT/ beta -actin ratios ± SEM in SN of 6 mice per group. (C) Double labeling of tyrosine hydroxylase (TH) and 3-NT in SN region of ventral midbrain. (D) Representative Western blots illustrating the expression of 4-HNE in SN. (E) Bar graph showing mean Western blot 4-HNE/ beta -actin ratios ± SEM in SN of 6 mice per group. (F) Double labeling of TH and 4-HNE in SN region of ventral midbrain. Images were captured at 60× magnification. The SN zone is outlined in white dots. Inset pictures demonstrated colocalization of TH and 3-NT/4-HNE. ***P <0.001 vs the control group; **P <0.01 vs the control group; *P <0.05 vs the MPTP group; #P <0.001 vs the MPTP group. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23092448), licensed under a CC-BY license. Not internally tested by R&D Systems.

    Western Blot Detection of Mouse 4-Hydroxynonenal by Western BlotView Larger

    Detection of Mouse 4-Hydroxynonenal by Western Blot Diapocynin inhibits the formation of 3-nitrotyrosine (3-NT) and 4-hydroxynonenal (4-HNE) in the substantia nigra (SN) of MPTP-treated mice. (A) Representative Western blots illustrating the expression of 3-NT in SN. (B) Bar graph showing mean Western blot 3-NT/ beta -actin ratios ± SEM in SN of 6 mice per group. (C) Double labeling of tyrosine hydroxylase (TH) and 3-NT in SN region of ventral midbrain. (D) Representative Western blots illustrating the expression of 4-HNE in SN. (E) Bar graph showing mean Western blot 4-HNE/ beta -actin ratios ± SEM in SN of 6 mice per group. (F) Double labeling of TH and 4-HNE in SN region of ventral midbrain. Images were captured at 60× magnification. The SN zone is outlined in white dots. Inset pictures demonstrated colocalization of TH and 3-NT/4-HNE. ***P <0.001 vs the control group; **P <0.01 vs the control group; *P <0.05 vs the MPTP group; #P <0.001 vs the MPTP group. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23092448), licensed under a CC-BY license. Not internally tested by R&D Systems.

    4-Hydroxynonenal Antibody Summary

    Specificity
    Detects 4-Hydroxynonenal adducts of histidine residues. No cross-reactivity with nitrotyrosine, formaldehyde, glutaraldehyde, or 4‑hydroxy hexenaldehyde adducts is observed.
    Source
    Monoclonal Mouse IgG2B Clone # 198960
    Purification
    Protein A or G purified from hybridoma culture supernatant
    Immunogen
    KLH-coupled 4-Hydroxynonenal
    Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
    Label
    Unconjugated

    Applications

    Recommended Concentration
    Sample
    Western Blot
    1 µg/mL
    See below
    Simple Western
    5 µg/mL
    See below
    Immunohistochemistry
    0.1-25 µg/mL
    See below

    Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

    组成成分
    IGG-免疫球蛋白G型抗体
    应用
    实验应用
    Western Blot(1 µg/mL)
    Simple Western(5 µg/mL)
    背景
    别名
    (2E)-4-Hydroxy-2-nonenal,2-Nonenal, 4-hydroxy-, (2E,4R)-,4 HNE,4Hydroxynonenal,4-Hydroxynonenal,4-hydroxy-trans-2-nonenal,HNE
    背景

    Background: 4-Hydroxynonenal

    4-hydroxy-2-nonenal (4-hydroxynonenal, 4-HNE) is a highly reactive aldehyde generated by the exposure of polyunsaturated fatty acids to peroxides and reactive oxygen species (ROS). It non-enzymatically forms stable protein adducts with histidine, lysine, and cysteine side chains that have been used as biomarkers for oxidative damage in cells. Conditions where 4-HNE immunoreactivity has been observed include include inflammation, neurodegenerative diseases, and ischemic damage to the heart and brain.

    Alternate Names
    (2E)-4-Hydroxy-2-nonenal ; 2-Nonenal, 4-hydroxy-, (2E,4R)-; 4 HNE; 4Hydroxynonenal; 4-Hydroxynonenal; 4-hydroxy-trans-2-nonenal; HNE
    研究领域
    制备和贮存
    溶解方法
    Reconstitute at 0.5 mg/mL in sterile PBS.
    保存方式

    Preparation and Storage

    Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
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    Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
    Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

    参考图片

    Detection of 4-Hydroxynonenal by Western Blot.
     Western blot shows recombinant E. coli DNAK treated (+) with 4-hydroxynonenal or peroxynitrite and lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated with 4-hydroxynonenal. PVDF membrane was probed with 1 µg/mL of Mouse Anti-4-Hydroxynonenal Monoclonal Antibody (Catalog # MAB3249), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

    Detection of Human 4‑Hydroxynonenal by Simple WesternTM.
     Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 4-Hydroxynonenal (4-HNE), loaded at 0.2 mg/mL. Specific bands were detected for 4‑Hydroxynonenal at approximately 4-Hydroxynonenal adducts of histidine residues kDa (as indicated) using 5 µg/mL of Mouse Anti-4‑Hydroxynonenal Monoclonal Antibody (Catalog # MAB3249). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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    货号：
    MAB3249
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    25ug
    100ug
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