8-oxo-dG Antibody (50 uL)

8-oxo-dG Antibody (50 uL)

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品牌: R&D Systems
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    来源宿主:
    Mouse IgG2b
    Mouse IgG2b
    实验应用:
    ELISA, Immunohistochemistry, Immunocytochemistry
    ELISA, Immunohistochemistry, Immunocytochemistry
    产品介绍
    产品信息
    耦联标记
    Unconjugated
    宿主
    Mouse IgG2b
    免疫原
    8-oxo-dG-conjugated-KLH
    克隆号
    15A3

    Scientific Data

    Immunocytochemistry/ Immunofluorescence Detection of Rat 8-oxo-dG by Immunocytochemistry/ ImmunofluorescenceView Larger

    Detection of Rat 8-oxo-dG by Immunocytochemistry/ Immunofluorescence Expression of apoptotic factors in denervated muscle 2 weeks after denervation.(A) Expression of Bcl-2, Bad, and Bax in denervated muscle subjected to different treatment (B) Quantitative analysis of apoptotic markers in different treatment groups (C) The expression of 8-oxo-dG in normal, control, ES, and AFS group. (D) The representative of western blot analysis of 8-oxo-dG related to different treatment (n = 2) (F) Quantitative analysis of caspase-3 in different treatment groups Bar length = 100 μm. R = right, L = left, N = 6 for each group, * p<0.05 and **p<0.01indicated a statistical difference compared to the control group; # p<0.05 indicated a statistical difference as compared to the ES group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25945496), licensed under a CC-BY license. Not internally tested by R&D Systems.

    8-oxo-dG Antibody Summary

    Specificity
    This mouse monoclonal antibody specifically binds to 8-hydroxy-2'- deoxyguanosine within DNA in H2O2-treated cells.
    Source
    Monoclonal Mouse IgG2B Clone # 15A3
    Purification
    Protein A or G purified from ascites
    Immunogen
    8-oxo-dG-conjugated-KLH
    Formulation
    This antibody is provided as purified immunoglobulin from mouse ascites at 0.5 mg/ml in 1X PBS containing 0.1% sodium azide, 50% glycerol.
    Label
    Unconjugated

    Applications

    Recommended Concentration
    Sample
    ELISA
    Empirical determination will be required for optimal results.
     
    Immunohistochemistry
    1:250 dilution
    Paraffin embedded rat thymus tissue
    Immunocytochemistry
    1:250 dilution

    H2O2 treated MCF 10A human breast epithelial cell line

    Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

    应用
    实验应用
    ELISA, Immunohistochemistry, Immunocytochemistry
    背景
    背景

    Background: 8-oxo-dG

    The production of 8-Hydroxyguanine (8-oxo-dG) is almost exclusively elicited by oxidative stress. Polymerases preferentially insert adenine opposite 8-oxo-dG. Therefore, oxidatively damaged adducts, without repair, are susceptible to G to T transitions.

    Long Name
    8-Hydroxyguanine
    Alternate Names
    8-OHG; 8oxodG; 8-oxo-dG
    研究领域
    制备和贮存
    保存方式

    Preparation and Storage

    Shipping
    The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage
    Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
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    货号:
    4354-MC-050
    一键复制
    询价
    50uL
    选择数量
    当前规格1件起购 
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