Acetylated-Lysine Antibody

Western blot analysis of extracts from NIH/3T3 cells, untreated or sodium butyrate-treated (5 mM for 24 hours), showing an increase in histone acetylation using Acetylated-Lysine Antibody.

对NIH/3T3细胞抽提液，未处理或5 mM丁酸钠处理24小时，使用Acetylated-Lysine Antibody进行Western blot分析，显示组蛋白乙酰化增加。

Western blot analysis of immunoprecipitated p53 showing an increase in p53 acetylation using Acetylated-Lysine Antibody (upper) or p53 antibody (lower). p53 was immunoprecipitated from lysates from 293 cells, untreated or UV-treated, using p53 Antibody #9282.

对免疫共沉淀的p53，使用Acetylated-Lysine Antibody（上图）或p53 antibody（下图）进行Western blot分析，显示p53乙酰化增加。从293细胞裂解后免疫共沉淀的p53，未处理或UV处理，使用p53 Antibody #9282。

Immunohistochemical staining of a paraffin-embedded human breast tumor section showing nuclear and cytoplasmic localization of proteins with acetylated lysine residues using Acetylated-Lysine Antibody.

对石蜡包埋的人乳腺肿瘤切片使用Acetylated-Lysine Antibody进行免疫组化分析，显示细胞核和细胞质定位。

Western blot analysis of extracts from COS cells, untreated or TSA-treated, grown in 10% FBS (lanes 1 and 2) or serum starved for 18 hours (lanes 3 and 4), using Acetylated-Lysine Antibody (upper) or p44/42 MAP Kinase Antibody #9102 (lower).

对COS细胞，未处理或TSA处理，在10% FBS（列1和列2）生长或血清饥饿处理18小时（列3和列4），使用Acetylated-Lysine Antibody（上图）或p44/42 MAP Kinase Antibody #9102（下图）进行Western blot分析。

Specificity and sensitivity of Acetylated-Lysine Antibody assayed on acetylated BSA (4; 1; 0.2; 0.04 or 0.008 ng in lanes 1-5) or nonacetylated BSA (25,000; 5,000; 1,000 or 200 ng in lanes 6-9).

Acetylated-Lysine Antibody的特异性或敏感性用乙酰化BSA（4、1、0.2、0.04或0.008 ng 列1-5）和非乙酰化BSA（25,000、5,000、1,000或200 ng 列6-9）进行检测。

Immunohistochemical analysis of paraffin-embedded NIH/3T3 untreated (left) or TSA-treated (right) using Acetylated-Lysine Antibody.

对NIH/3T3细胞，未处理（左）或TSA处理（右），使用Acetylated-Lysine Antibody进行免疫组化分析。

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Acetylated-Lysine Antibody.

对石蜡包埋的人结肠癌使用Acetylated-Lysine Antibody抗体进行免疫组化分析。

Confocal immunofluorescent analysis of NIH/3T3 cells, untreated (left) or SAHA-treated (right), labeled with Acetylated-Lysine Antibody (green). Actin filaments have been labeled with Alexa Fluor R 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

对NIH/3T3细胞，未处理（左）或SAHA处理（右），使用Acetylated-Lysine Antibody（绿）进行共聚焦免疫荧光分析。肌动蛋白用Alexa Fluor® 555 phalloidin（红色）标记。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HeLa cells and either 20 μl of Acetylated-Lysine Antibody or 2 μl of Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads) #9002. The enriched DNA was quantified by real-time PCR, using SimpleChIP® Human GAPDH Exon 1 Primers #5516, SimpleChIP® Human RPL30 Exon 3 Primers #7014, SimpleChIP® Human MyoD1 Exon 1 Primers #4490, and SimpleChIP® Human MYT-1 Exon 1 Primers #4493. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

使用SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads) #9002试剂盒，将4 x 10^6 HeLa细胞的染色质，与20μl Acetylated-Lysine (Ac-K2-100) Rabbit mAb #9814或者2 μl of Normal Rabbit IgG #2729进行染色质免疫沉淀。使用人SimpleChIP® Human GAPDH Exon 1 Primers #5516, SimpleChIP® Human RPL30 Exon 3 Primers #7014, SimpleChIP® Human MyoD1 Exon 1 Primers #4490和SimpleChIP® Human MYT-1 Exon 1 Primers #4493进行real-time PCR用来定量富集的DNA。每个样本中免疫沉淀的DNA使用相对于输入对照中染色质的量描述，输入对照的量相当于1。