




Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Immunohistochemistry (Paraffin) | 1:200 - 1:800 |
Immunofluorescence (Immunocytochemistry) | 1:400 - 1:1600 |
Flow Cytometry (Fixed/Permeabilized) | 1:200 - 1:800 |




Specificity/Sensitivity
物种反应性:
人, 猴






参考图片
Confocal immunofluorescent analysis of HT-29 cells using ASCT2 (D7C12) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
激光共聚焦免疫荧光检测HT-29细胞提取物,所用抗体ASCT2 (D7C12) Rabbit mAb兔单抗(绿色),蓝色伪彩为DNA荧光染料(对应产品号为DRAQ5® #4084)
Western blot analysis of extracts from HT-29 cells, untreated or treated with peptide N-glycosidase F (PNGase F), and untreated SW620 cells using ASCT2 (D7C12) Rabbit mAb.
Wetern blot检测HT-29细胞提取物,未处理或用N-糖苷酶F处理,以及未处理的SW620细胞。所用抗体为ASCT2 (D7C12) Rabbit mAb兔单抗。
Flow cytometric analysis of HT-29 cells using ASCT2 (D7C12) Rabbit mAb (blue) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).
流式细胞仪检测HT-29细胞,所用抗体为ASCT2 (D7C12) Rabbit mAb兔单抗 (绿色),对照组所用抗体为浓度匹配的Rabbit (DA1E) mAb IgG XP® Isotype Control #3900(红色)。