BD Transduction Laboratories™ Purified Mouse Anti-Actin Ab-5

Actin

Mouse IgG1, κ

Chicken gizzard muscle Actin

Changes in cellular morphology, adhesion, and motility occur through the reorganization of the actin cytoskeleton.  This reorganization of actin filaments results from interactions between actin and actin-binding proteins.  Actin is a 42-kDa protein that is known as G-actin in its monomeric form.  Polymerization of G-actin monomers leads to the generation of flexible filaments, 5-9 nm in diameter, called F-actin.  F-actin may be organized in linear bundles called stress fibers or in two-dimensional networks.  The latter are highly concentrated beneath the plasma membrane and form the actin cortex.  Regulation of actin cytoskeletal dynamics occurs through actin-binding proteins.  These proteins bind to G- and/or F-actin and regulate various aspects of actin cytoskeletal dynamics, such as polymerization and depolymerization of actin, cross-linking of actin filaments into bundles, interaction of actin-based structures with membranes and other cytoskeletal elements, and locomotion of actin-based structures.  Thus, the actin cytoskeleton is a complex matrix consisting of G- and F-actin along with the multitude of interactions between these actin forms and a variety of different types of actin-binding proteins. The C4 monoclonal antibody reacts with all known isoforms of actin in vertebrate muscle and non-muscle cells.

Changes in cellular morphology, adhesion, and motility occur through the reorganization of the actin cytoskeleton.  This reorganization of actin filaments results from interactions between actin and actin-binding proteins.  Actin is a 42-kDa protein that is known as G-actin in its monomeric form.  Polymerization of G-actin monomers leads to the generation of flexible filaments, 5-9 nm in diameter, called F-actin.  F-actin may be organized in linear bundles called stress fibers or in two-dimensional networks.  The latter are highly concentrated beneath the plasma membrane and form the actin cortex.  Regulation of actin cytoskeletal dynamics occurs through actin-binding proteins.  These proteins bind to G- and/or F-actin and regulate various aspects of actin cytoskeletal dynamics, such as polymerization and depolymerization of actin, cross-linking of actin filaments into bundles, interaction of actin-based structures with membranes and other cytoskeletal elements, and locomotion of actin-based structures.  Thus, the actin cytoskeleton is a complex matrix consisting of G- and F-actin along with the multitude of interactions between these actin forms and a variety of different types of actin-binding proteins. The C4 monoclonal antibody reacts with all known isoforms of actin in vertebrate muscle and non-muscle cells.

Mouse IgG1, κ

克隆 C4/actin (RUO)

250 µg/ml

Western blot (Routinely Tested), Immunofluorescence (Tested During Development)

Human (QC Testing), Mouse,Rat,Dog,Chicken (Tested in Development)

Actin

Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

研发参考(4) 1. Hanstein C, Lange U, Schneider-Poetsch HA, Grolig F, Wagner G. Detection of actin and localization of phytochrome in the green alga Mougeotia by monocloanl antibodies. Acta Histochem Suppl. 1991; 41:223-230. (Biology). 2. Lesssard JL. Two monoclonal antibodies to actin: one muscle selective and one generally reactive. Cell Motil Cytoskeleton. 1988; 10(3):349-362. (Biology). 3. Mitchison TJ, Cramer LP. Actin-based cell motility and cell locomotion. Cell. 1996; 84(3):371-379. (Biology). 4. Pantaloni D, Le Clainche C, Cartier M-F. Mechanism of Actin-Based Motility. Science. 2001; 292:1502-1506. (Biology).