Pan-Actin (D18C11) Rabbit mAb

Pan-Actin (D18C11) Rabbit mAb

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品牌: CST
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    分子量:
    45
    45
    反应种属:
    Human,Mouse,Rat,Monkey,
    Human,Mouse,Rat,Monkey,
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    产品介绍
    产品信息
    抗原名称
    Pan-Actin
    来源纯化
    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human β-actin protein.
    宿主
    Rabbit
    简单描述
    Monoclonal Antibody for studying beta-Actin/ACTA1 (alpha actin, skeletal muscle)/ACTG1 (gamma actin cytoplasmic nonmuscle)/ACTC1 (alpha cardiac actin)/ACTA2 (alpha actin, smooth muscle)/ACTG2 (gamma actin, cytoplasmic). Cited in 99 publications. Validated for WB, IHC, IF. Highly specific and rigorously validated in-house, Pan-Actin (D18C11) Rabbit Monoclonal Antibody (CST #8456) is ready to ship.
    商品描述

    Product Usage Information

    ApplicationDilution
    Western Blotting1:1000
    Immunohistochemistry (Paraffin)1:200
    Immunofluorescence (Immunocytochemistry)1:50
    分子量
    45
    研究领域
    癌症,细胞生物学,纤维化,神经科学,
    应用
    反应种属
    Human,Mouse,Rat,Monkey,
    目标/特异性

    Specificity/Sensitivity

    Pan-Actin (D18C11) Rabbit mAb recognizes endogenous levels of total actin protein (all isoforms).

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    敏感性
    Endogenous
    背景
    背景
    Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are ubiquitously expressed, controlling cell structure and motility (1). While all actin isoforms are highly homologous, cytoplasmic β- and γ-actin protein sequences differ by only four biochemically similar amino acids (2). For this reason, antibodies raised to β-actin may cross-react with γ-actin, and vice versa. α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (3). The ARP2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (3). Research studies have shown that actin is hyperphosphorylated in primary breast tumors (4). Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle, as shown in research studies (5-7). Actin cleavage by caspase-3 may accelerate ubiquitin/proteasome-dependent muscle proteolysis (7). 1.Herman, I.M. (1993) Curr. Opin. Cell Biol. 5, 48-55. 2.Perrin, B.J. and Ervasti, J.M. (2010) Cytoskeleton (Hoboken) 67, 630-4. 3.Condeelis, J. (2001) Trends Cell Biol 11, 288-93. 4.Lim, Y.P. et al. (2004) Clin Cancer Res 10, 3980-7. 5.Kayalar, C. et al. (1996) Proc Natl Acad Sci U S A 93, 2234-8. 6.Communal, C. et al. (2002) Proc Natl Acad Sci U S A 99, 6252-6. 7.Du, J. et al. (2004) J Clin Invest 113, 115-23.
    研究领域
    癌症,细胞生物学,纤维化,神经科学,
    翻译后修饰
    Unmodified
    制备和贮存
    保存方式
    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
    数据库链接
    Entrez-Gene ID
    60,58,71,70,59,72
    UniProt ID
    P60709,P68133,P63261,P68032,P62736,P63267

    参考图片

    Western blot analysis of extracts from various cell lines using Pan-Actin (D18C11) Rabbit mAb.使用Pan-Actin (D18C11)Rabbit mAb对多种细胞提取物进行western blot分析。

    Immunohistochemical analysis of paraffin-embedded human heart using Pan-Actin (D18C11) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).在对照肽(左)或抗原特异性肽(右)存在的条件下,使用Pan-Actin (D18C11)Rabbit mAb对石蜡包埋的人乳腺癌组织进行免疫荧光分析。

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Pan-Actin (D18C11) Rabbit mAb.使用Pan-Actin (D18C11)Rabbit mAb对石蜡包埋的人结肠癌组织进行免疫组化分析。

    Confocal immunofluorescent analysis of HeLa cells using Pan-Actin (D18C11) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用Pan-Actin (D18C11)Rabbit mAb(绿色)对HeLa细胞进行激光共聚焦免疫荧光分析。蓝色假色= DRAQ5® #4084 (fluorescent DNA dye).

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    货号:
    8456S
    一键复制
    询价
    供应商现货
    20μl
    100ul
    选择数量
    当前规格1件起购 
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    预计5-6个工作日送达,快递: 免运费,若需干冰额外收费