AffiniPure™ F(ab‘)2 Fragment Goat Anti-Human IgG, Fcγ Fragment Specific (min X Bov,Hrs,Ms Sr Prot)

AffiniPure™ F(ab‘)2 Fragment Goat Anti-Human IgG, Fcγ Fragment Specific (min X Bov,Hrs,Ms Sr Prot)

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品牌: Jackson
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    反应种属:
    Human
    Human
    产品介绍
    产品信息
    抗原名称
    IgG, Fcγ Fragment

    Target: Human
    Host: Goat
    Antibody Format: F(ab')₂ Fragment
    Specificity: IgG, Fcγ fragment specific
    Minimal Cross Reactivity: Bovine, Horse, Mouse Serum Proteins
    Conjugate: Unconjugated
    Product Category: F(ab')₂ Fragment Affinity-Purified Antibodies
    Clonality: Polyclonal
    RRID: AB_2337551

    Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of human IgG heavy chain but not with the Fab portion of human IgG. No antibody was detected against human IgM or IgA, or against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, horse and mouse serum proteins, but it may cross-react with immunoglobulins from other species.

    F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.

    Physical State: Sterile-filtered liquid

    Storage: Store at 2-8°C under sterile conditions. Prepare working dilution on day of use.
    Expiration date: one year from date of receipt. The expiration date may be extended if test results are acceptable for the intended use.


    Purity: The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
    Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
    Preservative: None

    Suggested Working Concentration or Dilution Range:

    10-20 µg / ml

    Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

    组成成分
    山羊抗人免疫球蛋白G抗体;缓冲液(0.01M磷酸钠、0.25M氯化钠)
    应用
    反应种属
    Human
    交叉反应性
    Bovine, Horse, Mouse Serum Proteins
    最小交叉反应
    Bovine, Horse, Mouse Serum Proteins
    目标/特异性
    IgG, Fcγ fragment specific
    数据库链接
    研究资源识别码
    AB_2337551
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    货号:
    109-006-098
    一键复制
    询价
    1mg
    选择数量
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