Akt2 (1G8C12)

mouse

Akt2抗体(1G8C12)是小鼠单克隆IgG2b (kappa轻链)，规格为200 µg/ml raised against a recombinant protein corresponding to amino acids 2-150 of Akt2 of human origin 抗-Akt2 抗体 (1G8C12) 推荐用于 WB 和 IP，检测mouse, rat 和human 来源的 Akt2 抗-Akt2 抗体 (1G8C12) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统 关于如何获取Akt2 (1G8C12): sc-81148的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。 m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 Akt2 Antibody (1G8C12) for WB 首选应用二抗. 这些试剂现在以套形式提供 Akt2 Antibody (1G8C12) (见下方订购信息). The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKBβ or RacPK-β) and Akt 3 (also designated PKBγ or thyoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a Pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation that is dependent on PDGFR-β tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s), and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin. Taken together, this data strongly suggests that the protein signals downstream of the PI kinases. Akt3 is phosphorylated on a serine residue in response to insulin, and this activation is inhibited by prior activation of protein kinase C. Akt3 is expressed in 3T3-L1 fibroblasts, adipocytes and skeletal muscle and may be involved in various biological processes, including adipocyte and muscle di

Replacement monoclonal antibody for: sc-7127.

IGG-免疫球蛋白G型抗体

HumanAKT2

208;11652