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Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb

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Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb

品牌： CST

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参数

分子量：

反应种属：

Human,Mouse,Rat,Monkey,

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产品介绍

产品信息

抗原名称

PRAS40

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequence surrounding Thr246 of human PRAS40.

宿主

Rabbit

简单描述

Monoclonal Antibody for studying PRAS40 (Thr246) phosphate. Cited in 211 publications. Validated for WB, WB, IP, IHC. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-PRAS40 (Thr246) (C77D7) Rabbit Monoclonal Antibody (CST #2997) is ready to ship.

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Simple Western™	1:10 - 1:50
Immunoprecipitation	1:50
Immunohistochemistry (Paraffin)	1:800 - 1:3200

分子量

研究领域

癌症,细胞生物学,纤维化,代谢,神经科学

应用

反应种属

Human,Mouse,Rat,Monkey,

目标/特异性

Specificity/Sensitivity

Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb detects endogenous levels of PRAS40 protein only when phosphorylated at Thr246.

Species Reactivity:

Human, Mouse, Rat, Monkey

敏感性

Endogenous

背景

Many growth factors and hormones induce the phosphoinositide 3-kinase signaling pathway, which results in the activation of downstream effector proteins such as the serine/threonine kinase Akt (1,2). One known Akt substrate is a 40 kDa, proline-rich protein (PRAS40) that binds to 14-3-3 proteins (2). PRAS40 also binds mTOR to transduce Akt signals to the mTOR complex. Inhibition of mTOR signaling stimulates PRAS40 binding to mTOR, which in turn inhibits mTOR activity (3). PRAS40 interacts with raptor in mTOR complex 1 (mTORC1) in insulin-deprived cells and inhibits the activation of the mTORC1 pathway mediated by the cell cycle protein Rheb. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1 (4). mTORC1 in turn phosphorylates PRAS40 at Ser183 (5). 1.Cantley, L.C. (2002) Science 296, 1655-7. 2.Kovacina, K.S. et al. (2003) J Biol Chem 278, 10189-94. 3.Vander Haar, E. et al. (2007) Nat Cell Biol 9, 316-23. 4.Sancak, Y. et al. (2007) Mol Cell 25, 903-15. 5.Oshiro, N. et al. (2007) J Biol Chem 282, 20329-39.

研究领域

癌症,细胞生物学,纤维化,代谢,神经科学

翻译后修饰

Phosphate

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #60202.

数据库链接

Entrez-Gene ID

84335

UniProt ID

Q96B36

参考图片

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, control (left) or λ phosphatase-treated (right), using Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb.

免疫组织化学方法检测石蜡包埋的人乳腺癌组织，对照（左图）和λ磷酸酶处理（右图）；使用的抗体是Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb兔单抗。

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb in the presence of control peptide (left) or antigen specific peptide (right).

免疫组织化学方法检测石蜡包埋的人乳腺癌组织，对照肽处理（左图）和抗原特异性肽处理（右图）；使用的抗体是Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb兔单抗。

Immunohistochemical analysis of paraffin-embedded metastatic SKOV-3 tumor in mouse lung using Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb.

免疫组织化学方法检测石蜡包埋的鼠肺转移的SKOV-3肿瘤组织，使用的抗体是Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb兔单抗。

Western blot analysis of extracts from serum starved HeLa cells, untreated or treated with insulin (100 nM, 5 minutes) or with insulin and λ phosphatase, using Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb (upper) or PRAS40 Antibody #2610 (lower).

Western blot检测分析血清饥饿的Hela细胞提取物。未处理和胰岛素 (100 nM）处理5分钟或胰岛素和 λ磷酸酶处理的；使用抗体是Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb 兔单抗(上图)和 PRAS40 Antibody #2610 (下图)。

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货号：

2997T

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