首页抗体二抗抗豚鼠

Alexa Fluor® 488-conjugated AffiniPure™ Donkey Anti-Guinea Pig IgG (H+L) (min X Bov,Ck,Gt,Sy Hms,Hrs,Hu,Ms,Rb,Rat,Shp Sr Prot)

品牌： Jackson

下载产品说明书

下载SDS

用小程序，查商品更便捷

对比

咨询

参数

反应种属：

Guinea Pig

来源宿主：

驴

产品介绍

产品信息

抗原名称

IgG (H+L)

宿主

驴

Target: Guinea Pig
Host: Donkey
Antibody Format: Whole IgG
Specificity: IgG (H+L)
Minimal Cross Reactivity: Bovine, Chicken, Goat, Syrian Hamster, Horse, Human, Mouse, Rabbit, Rat, Sheep Serum Proteins
Conjugate: Alexa Fluor® 488
Product Category: Whole IgG Affinity-Purified Antibodies
Clonality: Polyclonal
RRID: AB_2340472

Information

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule guinea pig IgG. It also reacts with the light chains of other guinea pig immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, syrian hamster, horse, human, mouse, rabbit, rat and sheep serum proteins, but it may cross-react with immunoglobulins from other species.

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Usage

Physical State: Freeze-dried solid

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid.
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative: 0.05% Sodium Azide

Suggested Working Concentration or Dilution Range:

1:100 - 1:800 for most applications

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Conjugate

Alexa Fluor® 488

Amax: 493 Emax: 519nm

Alexa Fluor® 488-conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media they are brighter than FITC, Cy2, and DyLight 488. Alexa Fluor® 488 conjugates are recommended for maximum sensitivity for all immunofluorescence procedures requiring a green-fluorescing dye, except for protocols that include mounting in plastic mounting media.

组成成分

488荧光染料偶联驴抗豚鼠免疫球蛋白G抗体;缓冲液(0.01M磷酸钠、0.25M氯化钠);稳定剂(1.5%牛血清白蛋白);防腐剂(0.05%叠氮化钠溶液)

应用

反应种属

Guinea Pig

交叉反应性

Bovine, Chicken, Goat, Syrian Hamster, Horse, Human, Mouse, Rabbit, Rat, Sheep Serum Proteins

最小交叉反应

Bovine, Chicken, Goat, Syrian Hamster, Horse, Human, Mouse, Rabbit, Rat, Sheep Serum Proteins

目标/特异性

IgG (H+L)

数据库链接

研究资源识别码

AB_2340472

声明：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。

货号：

706-545-148

一键复制

询价

0.5mg

选择数量

当前规格1件起购

配送至

预计2-3周送达，快递: 免运费，若需干冰额外收费

询价大包装询价