Alexa Fluor® 647 Antibody Labeling Kit

Ideal antibody concentration is 5 mg/ml can be used. Antibodies > 5 mg/ml or < 2 mg/ml should be diluted /concentrated.
The recommended buffer system for antibodies is only PBS, pH 7.2-7.4.

ICC shows positive staining in HeLa cells. Anti-Vimentin (Alexa Fluor® 647 Conjugate) antibody (S0B0146) was used at 1/250 dilution (magenta) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

IF shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti-alpha smooth muscle Actin (Alexa Fluor® 647 Conjugate) antibody (S0B0119) was used at 1/1000 dilution (magenta). Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

IF shows positive staining in paraffin-embedded human leiomyosarcoma. Anti-alpha smooth muscle Actin (Alexa Fluor® 647 Conjugate) antibody (S0B0119) was used at 1/1000 dilution (magenta). Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

IF shows positive staining in paraffin-embedded human colon cancer. Anti-Vimentin (Alexa Fluor® 647 Conjugate) antibody (S0B0146) was used at 1/250 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

Flow cytometric analysis of mouse primary splenocytes labeling CD8α (Alexa Fluor® 647 Conjugate) antibody (S0B0125) at 1/200 (1 μg) dilution / (right panel) compared with a Rabbit IgG, Isotype Control / (left panel). Cells were surface stained with CD4-APC-Cy7, then stained with Rabbit IgG (Left) / CD8α (Right) separately. CD4 and CD8α are mutually exclusive expressed in mouse primary splenocytes. Gated on total viable cells.