Alexa Fluor® 790-conjugated AffiniPure™ Goat Anti-Mouse IgG (H+L) (min X Hu,Bov,Hrs,Rb,Sw Sr Prot)

Alexa Fluor® 790-conjugated AffiniPure™ Goat Anti-Mouse IgG (H+L) (min X Hu,Bov,Hrs,Rb,Sw Sr Prot)

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品牌: Jackson
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    反应种属:
    Mouse
    Mouse
    产品介绍
    产品信息
    抗原名称
    IgG (H+L)

    Target: Mouse
    Host: Goat
    Antibody Format: Whole IgG
    Specificity: IgG (H+L)
    Minimal Cross Reactivity: Human, Bovine, Horse, Rabbit, Swine Serum Proteins
    Conjugate: Alexa Fluor® 790
    Product Category: Near-InfraRed (NIR) Fluorescent Conjugates
    Clonality: Polyclonal
    RRID: AB_2338944

    Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG. It also reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine, horse, rabbit and swine serum proteins, but it may cross-react with immunoglobulins from other species.

    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

    Physical State: Freeze-dried solid

    Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
    Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid.
    Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.


    Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
    Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
    Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
    Preservative: 0.05% Sodium Azide

    Suggested Working Concentration or Dilution Range:

    Western Blot:- 1:50,000-200,000

    Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

    Alexa Fluor® 790

    Amax: 792 Emax: 803nm

    Alexa Fluor® 680 and Alexa Fluor® 790 conjugates are used for very sensitive Western blots, ELISAs, and multiplexing arrays. Alexa Fluor® 680 conjugates are excited with a peak around 684 nm and fluoresce with a peak around 702 nm. Alexa Fluor® 790 conjugates are excited with a peak around 792 nm and fluoresce at a peak around 803 nm. They are the best choice for highly sensitive single or double labeling with fluorescence imaged in a LI-COR Odyssey® imager.

    组成成分
    790荧光染料偶联山羊抗小鼠免疫球蛋白G抗体;缓冲液(0.01M磷酸钠、0.25M氯化钠);稳定剂(1.5%牛血清白蛋白);防腐剂(0.05%叠氮化钠溶液)
    应用
    反应种属
    Mouse
    交叉反应性
    Human, Bovine, Horse, Rabbit, Swine Serum Proteins
    最小交叉反应
    Human, Bovine, Horse, Rabbit, Swine Serum Proteins
    目标/特异性
    IgG (H+L)
    数据库链接
    研究资源识别码
    AB_2338944
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    货号:
    115-655-146
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    询价
    0.5mg
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