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Anti-STREPTAVIDIN (RABBIT) Antibody Biotin Conjugated (BULK ORDER) - 200-4695


品牌: Rockland








纯度:
Streptavidin Antibody Biotin Conjugated was prepared from monospecific antiserum by delipidation, defibrination, salt fractionation and ion exchange chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum and Streptavidin. No reaction was observed against Avidin.
Streptavidin Antibody Biotin Conjugated was prepared from monospecific antiserum by delipidation, defibrination, salt fractionation and ion exchange chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum and Streptavidin. No reaction was observed against Avidin.
反应种属:
Streptavidin
Streptavidin
产品介绍
产品信息
耦联标记
Biotin

宿主
Rabbit

免疫原
Streptavidin (Streptomyces avidinii)

简单描述
Anti-Streptavidin (RABBIT) Antibody Biotin Conjugated (BULK ORDER) - 200-4695

同种型
IgG

克隆类别
Polyclonal

纯度
Streptavidin Antibody Biotin Conjugated was prepared from monospecific antiserum by delipidation, defibrination, salt fractionation and ion exchange chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum and Streptavidin. No reaction was observed against Avidin.

防腐剂
0.01% (w/v) Sodium Azide

应用
实验应用
Dot Blot

反应种属
Streptavidin

样本类型以及上样量
1.0 mL

背景
别名
rabbit anti-Streptavidin Antibody biotin Conjugation, biotin conjugated rabbit anti-Streptavidin antibody, Anti-Streptavidin BAC Antibody

背景
Anti-Streptavidin Antibody is Biotin Conjugated and detects streptavidin. Biotin is widely used throughout the biotechnology industry to conjugate proteins for biochemical assays. Biotin's small size typically does not affect the biological activity of protein upon biotinylation. Streptavidin and avidin bind biotin with high affinity (Kd of 10−14 mol/l to 10−15 mol/l) and thus biotinylated proteins of interest can be enriched from a sample due to this highly stable interaction. Biotin conjugated anti-streptavidins are used as an amplifying reagent in immunohistochemistry, microarray assays, ELISAs, blots, and other applications. This antibody reagent can bind to streptavidin through the antibody F(ab) or can be bound by streptavidin through the high affinity biotin-streptavidin interaction.

制备和贮存
存储溶液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

保存方式
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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