Anti-Z-DNA/Z-RNA [Z22], Mouse IgG2b, kappa

Z-22; Z 22; Z DNA; ZDNA; Z RNA; ZRNA; Z NA; Z-NA; ZNA Immunogen: Z22 was prepared by immunizing C57BL/6 mice with brominated poly(dG-dC).poly(dG-dC) complexed with methylated bovine serum albumin (BSA), and was selected for by ELISA. Brominated poly(dG-dC).poly(dG-dC) forms a stable Z-DNA helix under physiological salt conditions.

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Z22 binding to Z-DNA was evaluated by a competitive solid-phase ELISA (Möller et al., 1982; PMID: 7118931). The binding affinity of Z22 Fab version engineered from the original mouse IgG2b format to Z-DNA by SPR was measured to have an apparent KD of ~160 nM and could be competed out by soluble brominated d(G-C)15 but not unmodified d(G-C)15 (B-DNA). Z22 scFv binds to the target with a similar affinity to Z22 Fab. Competitive ELISA was used to determine binding specificity. Gel retardation assays were also used to show Z22 binding to DNA-8-MOP adducts. Biotinylated Z22 was used to determine the distribution of Z-DNA in permeabilized, microbead-encapsulated nuclei after adding radioactive streptavidin, and compared to encapsulated permeabilized nuclei stained with DAPI (Wittig et al., 1989; PMID: 2921282). Z22 was shown to cross-react with Z-RNA (Zarling et al., 1990; PMID: 2153833). Z-RNA binding using this product (Ab00783-3.0) was shown using IF (Zhang et al., 2020; PMID: 32200799), and it was also used in FC (Koehler et al., 2021; PMID: 34192517). Furthermore, this product (Ab00783, unspecified isotype) was also used in IHC and IB (Yau et al., 2021; PMID: 34728780), and Electrophoretic Mobility Shift Assay (EMSA; gel mobility assay) (Li et al., 2022; PMID: 35744832).

Recombinant monoclonal antibody to Z-DNA/Z-RNA. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma Z22.

Z22

1 mg/ml.

左旋脱氧核糖核酸抗体，磷酸盐缓冲液

EMSA, gel retardation assay, IB, in vitro, SPR, ELISA, FC, IF, IHC

Species independent

Z22 binds both Z-DNA, but not B-DNA or ssDNA (single-stranded DNA). It recognizes Z-DNA at the phosphodiester backbone of various base sequence including (dG-dC)n.(dG-dC)n, (dTdG)n.(dC-dA)n, (dG-dme5C)n.(dG-dme5C)n and (dG-dbr5C)n.(dG-dbr5C)n. In other words, Z22 binds Z-DNA irrespective of sequence. Z22 Also binds DNA-8-MOP aducts and Z-RNA.