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多因子、高通量筛选之Antibody Array抗体芯片

时间:2016-11-21 06:52:16 浏览次数:1425

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样本珍贵,不易得,想多检测几个因子,量不够,怎么办?
想检测的因子太多,不想一次次的重复试验,怎么办?
经费太少,还没怎么用,就快没了,怎么办?
 
 
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多因子、高通量筛选 之Antibody Array芯片平台 

 
▍抗体芯片平台介绍:
 
优宁维代理的R&Dsystems的抗体芯片是ELISA原理的高通量WB,每个芯片在设计时都利用了精心挑选的捕获抗体,它们在硝酸纤维素膜上重复点样两次。

通过监控蛋白水平和一些蛋白翻译后修饰的变化,能迅速靶定目标分子,以便进一步的研究。R&DSystems的蛋白芯片能广泛地应用于信号转导,血管再生,细胞凋亡,肿瘤,肥胖,以及干细胞,药物筛选等的研究。

蛋白芯片能同时检测单个样品中多种分析物的相对水平。蛋白芯片的使用不需要任何特殊的仪器,也取代了多次免疫沉淀/ Western blot实验,蛋白芯片高通量定性分析试剂盒比WB的灵敏度高100倍;更有大规模多分子高通量筛选作用,经多项研究证实,蛋白芯片能简化鉴定蛋白所需的筛选过程。

▍抗体芯片特点:


      ◆ 多样性:一张膜可检测15-119个目标蛋白;
     ◆ 高效率:所需样品量少,大大缩短操作时间,有效节约成本;
     ◆ 操作简单:即用型产品,无需购买抗体,无需特殊仪器
     ◆ 高品质:高特异性和低批间差距保证样品的检测结果的稳定性; 

▍抗体芯片实验流程: 

 
▍抗体芯片和WB比较如下:
 
 
  WB ARRAY
结果 定性 定性
通量 10-20 data points/membrane 15-119 data points/membrane(根据膜种类而定)
灵敏度
操作时间 5-6h + Overnight 2.5-3h+ Overnight
工作量:
8 samples*20因子
20 membranes 8 membranes


▍部分芯片产品清单,若需要详细清单请联系我们
 
ARY001B: human phospho-RTK
1×4 membranes
Sample Type:cell lysate
ALK/CD246 EphA5 ErbB3 M-CSF R SCF R/c-kit
Axl EphA6 ErbB4 Mer Tie-1
DDR1 EphA7 FGF R1 MSP R/Ron Tie-2
DDR2 EphA10 FGF R2 alpha MuSK TrkA
Dtk EphB1 FGF R3 PDGF R alpha TrkB
EGF R EphB2 FGF R4 PDGF R beta TrkC
EphA1 EphB3 Flt-3/Flk-2 c-Ret VEGF R1/Flt-1
EphA2 EphB4 HGF R/c-MET ROR1 VEGF R2/KDR
EphA3 EphB6 IGF-I R ROR2 VEGF R3/Flt-4
EphA4 ErbB2 Insulin R/CD220 Ryk  
ARY002B: human phospho-MAPK
1×4 membranes
Sample Type: cell lysate
Akt1 ERK2 JNK3 p38 beta RSK2
Akt2 GSK-3 alpha/beta JNK pan p38 delta TOR
Akt3 GSK-3 beta MKK3 p38 gamma  
Akt pan HSP27 MKK6 p53  
CREB JNK1 MSK2 p70 S6K  
ERK1 JNK2 p38 alpha RSK1  
ARY003B: human phospho-kinase
2×4 membranes
Sample Type: cell lysate
Akt 1/2/3 (S473) eNOS (S1177) JNK 1/2/3 (T183/Y185, T221/Y223) p70 S6 Kinase (T421/S424) STAT3 (Y705)
Akt 1/2/3 (T308) ERK1/2 (T202/Y204, T185/Y187) Lck (Y394) PDGF R beta (Y751) STAT5a (Y699)
AMPK alpha1 (T183) FAK (Y397) Lyn (Y397) PLC gamma-1 (Y783) STAT5a/b (Y699)
AMPK alpha2 (T172) Fgr (Y412) MSK1/2 (S376/S360) PRAS40 (T246) STAT5b (Y699)
beta-Catenin Fyn (Y420) p27 (T198) Pyk2 (Y402) STAT6 (Y641)
Chk-2 (T68) GSK-3 alpha/beta (S21/S9) p38 alpha (T180/Y182) RSK1/2/3 (S380) TOR (S2448)
c-Jun (S63) Hck (Y411) p53 (S15) Src (Y419) WNK-1 (T60)
CREB (S133) HSP27 (S78/S82) p53 (S392) STAT2 (Y689) Yes (Y426)
EGF R (Y1068) HSP60 p53 (S46) STAT3 (S727)  
ARY004B: human phospho-immunoreceptor
1×4 membranes
Sample Type: cell lysate
2B4/SLAMF4 CRACC/SLAMF7 ILT2/CD85j LMIR6/CD300LE Siglec-2/CD22
BLAME/SLAMF8 CTLA-4/CD152 ILT3/CD85k MDL-1/CLEC5A Siglec-3/CD33
BTLA DCIR/CLEC4A ILT4/CD85d NKp30/NCR3 Siglec-5
CD3 epsilon Dectin-1/CLEC7A ILT5/CD85a NKp44/NCR2 Siglec-7
CD5 DNAM-1 ILT6/CD85e NKp46/NCR1 Siglec-9
CD6 Fc epsilon RII/CD23 Integrin beta 3/CD61 NKp80/KLRF1 Siglec-10
CD28 Fc gamma RIIA KIR2DL4 NTB-A/SLAMF6 SIRP-beta 1
CD84/SLAMF5 Fc gamma RIIIA/B LAIR-1 PD-1 SLAM/CD150
CD229/SLAMF3 FcRH1/IRTA5 LAIR-2 PECAM/CD31 TREM-1
CEACAM-1 FcRH2/IRTA4 LMIR1/CD300A SHIP-1 TREM-2
CLEC-1 FcRH4/IRTA1 LMIR2/CD300C SHP-1 TREML1/TLT-1
CLEC-2 FcRH5/IRTA2 LMIR3/CD300LF SHP-2  
ARY005: human cytokine
1×4 membranes
Sample Type: cell culture supernatant, cell lysate, tissue homogenate, serum, plasma
C5a IL-1 alpha IL-10 IL-32 alpha CXCL12/SDF-1
CD40 ligand IL-1 beta IL-12 p70 CXCL10/IP-10 Serpin E1/PAI-1
G-CSF IL-1ra IL-13 CXCL11/I-TAC TNF-alpha
GM-CSF IL-2 IL-16 CCL2/MCP-1 TREM-1
CXCL1/GRO alpha IL-4 IL-17 MIF  
CCL1/I-309 IL-5 IL-17E CCL3/MIP-1 alpha  
ICAM-1 IL-6 IL-23 CCL4/MIP-1 beta  
IFN-gamma IL-8 IL-27 CCL5/RANTES   
ARY006: mouse cytokine
1×4 membranes
Sample Type: cell culture supernatant, cell lysate, tissue homogenate, serum, plasma
CXCL13/BLC/BCA-1 IL-1 alpha IL-10 CXCL11/I-TAC CXCL2/MIP-2
C5a IL-1 beta IL-12 p70 CXCL1/KC CCL5/RANTES
G-CSF IL-1ra IL-13 M-CSF CXCL12/SDF-1
GM-CSF IL-2 IL-16 CCL2/JE/MCP-1 CCL17/TARC
CCL1/I-309 IL-3 IL-17 CCL12/MCP-5 TIMP-1
CCL11/Eotaxin IL-5 IL-23 CXCL9/MIG TNF-alpha
ICAM-1 IL-6 IL-27 CCL3/MIP-1 alpha TREM-1
IFN-gamma IL-7 CXCL10/IP-10 CCL4/MIP-1 beta  
ARY007: human angiogenesis
1×4 membranes
Sample Type: cell culture supernatant, cell lysate, tissue homogenate, serum, plasma, saliva
Activin A EGF HB-EGF MMP-8 Serpin B5/Maspin
ADAMTS-1 EG-VEGF HGF MMP-9 Serpin E1/PAI-1
Angiogenin Endoglin/CD105 IGFBP-1 NRG1-beta 1 Serpin F1/PEDF
Angiopoietin-1 Endostatin/Collagen XVIII IGFBP-2 Pentraxin 3 TIMP-1
Angiopoietin-2 Endothelin-1 IGFBP-3 PD-ECGF TIMP-4
Angiostatin/Plasminogen FGF acidic IL-1 beta PDGF-AA Thrombospondin-1
Amphiregulin FGF basic CXCL8/IL-8 PDGF-AB/PDGF-BB Thrombospondin-2
Artemin FGF-4 LAP (TGF-beta 1) Persephin uPA
Tissue Factor/Factor III FGF-7/KGF Leptin CXCL4/PF4 Vasohibin
CXCL16 GDNF CCL2/MCP-1 PlGF VEGF
DPPIV/CD26 GM-CSF CCL3/MIP-1 alpha Prolactin VEGF-C

▍订货信息:

 
货号 产品名称 规格
ARY001B Proteome Profiler Human Phospho-RTK Array Kit (1 KT) 1 Kit(4张膜)
ARY002B Proteome Profiler Human Phospho-MAPK Array Kit (1 KT) 1 Kit(4张膜)
ARY003B Proteome Profiler Human Phospho-Kinase Array Kit (1 KT) 1 Kit(4套,每套2张膜PartA+PartB)
ARY004B Proteome Profiler Human Phospho-Immunoreceptor Array Kit,NEW (1 KT) 1 Kit(4张膜)
ARY005B Proteome Profiler Human Cytokine Array Kit, Panel A (1 KT) 1 Kit(4张膜)
ARY006 Proteome Profiler Mouse Cytokine Array Kit, Panel A (1 KT) 1 Kit(4张膜)
ARY007 Proteome Profiler Human Angiogenesis Array Kit (1 KT) 1 Kit(4张膜)
ARY008 Proteome Profiler Rat Cytokine Array Kit, Panel A (1 KT) 1 Kit(4张膜)
ARY009 Proteome Profiler Human Apoptosis Array Kit (1 KT) 1 Kit(4张膜)
ARY010 Proteome Profiler Human Pluripotent Stem Cell Array Kit (1 KT) 1 Kit(8张膜)
ARY011 Proteome Profiler Human sReceptor Array, Hematopoietic (1 KT) 1 Kit(4张膜)
ARY012 Proteome Profiler Human sReceptor Array, Non-hematopoietic (1 KT) 1 Kit(4张膜)
ARY013 Proteome Profiler Mouse Adipokine Array Kit (1 KT) 1 Kit(4张膜)
ARY014 Proteome Profiler Mouse Phospho-RTK Array Kit (1 KT) 1 Kit(4张膜)
ARY015 Proteome Profiler Mouse Angiogenesis Array Kit (1 KT) 1 Kit(4张膜)
ARY016 Proteome Profiler Rat Adipokine Array Kit (1 KT) 1 Kit(4张膜)
ARY017 Proteome Profiler Human Chemokine Array Kit (1 KT) 1 Kit(4张膜)
ARY018 Proteome Profiler Human Cell Stress Array Kit (1 KT) 1 Kit(4张膜)
ARY019 Proteome Profiler Human Kidney Biomarker Array Kit (1 KT) 1 Kit(4张膜)
ARY020 Proteome Profiler Mouse Chemokine Array Kit (1 KT) 1 Kit(4张膜)
ARY021B Proteome Profiler Human Protease Array Kit (1 KT) 1 Kit(4张膜)
ARY022 Proteome Profiler Human XL Cytokine Array Kit (1 KT) 1 Kit(4张膜)
ARY023 Proteome Profiler Human Protease Inhibitor Array Kit (1 KT) 1 Kit(4张膜)
ARY024 Proteome Profiler Human Adipokine Array Kit (1 KT) 1 Kit(4张膜)
ARY025 Proteome Profiler Human Protease/Protease Inhibitor Array (1 KT) 1 Kit(4张膜)
ARY026 Proteome Profiler Human XL Oncology Array (1 KT) 1 Kit(4张膜)
ARY027 Proteome Profiler Human Ubiquitin Array (1 KT) 1 Kit(4张膜)
ARY028 Proteome Profiler Mouse XL Cytokine Array (1 KT) 1 Kit(4张膜)
ARY029 Proteome Profiler Human NFkB Pathway Array Kit (1 KT) 1 Kit(4张膜)

 
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