外泌体产品线总攻略—小优助手来帮您!
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外泌体是细胞经过"内吞-融合-外排"等一系列调控过程而形成的细胞外纳米级(直径为30-100nm)小囊泡。外泌体可以携带蛋白,运送RNA,在细胞间物质和信息转导中起重要作用。外泌体可能通过调控免疫功能,促进肿瘤血管新生和肿瘤转移,以及直接作用于肿瘤细胞等途径,影响肿瘤的进展。外泌体由细胞分泌释放出来,在血液等体液内传播,最后又可被其他细胞吞噬,是细胞间通讯的重要介质。
外泌体目前被视为特异性分泌的膜泡,参与细胞间通讯,对外泌体的研究兴趣日益增长,从外泌体研究方向而言,干细胞、免疫、microRNA、靶向给药等都是热门研究领域,2013年诺贝尔生物/医学奖授予三位在细胞囊泡方面贡献突出的科学家,使外泌体的研究达到全新的高度,外泌体将在癌症的诊断及治疗等领域发挥重要作用。
Biovision公司则是提供了最全的外泌体解决方案:
1 流式检测试剂盒:检测原理如下:
产品清单:
产品编号 | 产品名称 | 产品规格 |
K1236-20 | ExoFACS™ Kit for Cell Culture Media Exosomes | 20 reactions |
K1232-20 | ExoFACS™ Kit for Plasma Exosomes | 20 reactions |
K1234-20 | ExoFACS™ Kit for Saliva Exosomes | 20 reactions |
K1233-20 | ExoFACS™ Kit for Serum Exosomes | 20 reactions |
K1235-20 | ExoFACS™ Kit for Urine Exosomes | 20 reactions |
2.外泌体定量试剂盒:适用不同样本类型;仅需100ul样本;采用夹心法ELISA技术。
产品清单:
产品编号 | 产品名称 | 产品规格 | 样本量和显色方法 |
K1205-100 | ExoQuant™ Overall Exosome Capture and Quantification Assay Kit | 100assays | Cell media, Colorimetri |
K1210-100 | ExoQuant™ Overall Exosome Capture and Quantification Assay Kit | 100assays | cell media, Luminometric |
K1201-100 | ExoQuant™ Overall Exosome Capture and Quantification Assay Kit | 100assays | human plasma, Colorimetric |
K1206-100 | ExoQuant™ Overall Exosome Capture and Quantification Assay Kit | 100assays | human plasma, Luminometric |
K1203-100 | ExoQuant™ Overall Exosome Capture and Quantification Assay Kit | 100assays | human serum, Colorimetric |
K1208-100 | ExoQuant™ Overall Exosome Capture and Quantification Assay Kit | 100assays | human serum, Luminometric |
K1202-100 | ExoQuant™ Overall Exosome Capture and Quantification Assay Kit | 100assays | human urine, Colorimetric |
K1207-100 | ExoQuant™ Overall Exosome Capture and Quantification Assay Kit | 100assays | human urine, Luminometric |
K1204-100 | ExoQuant™ Tumor-derived Exosome enrichment and Quantification Assay Kit |
100assays | biological fluids/cell media, Colorimetric |
K1209-100 | ExoQuant™ Tumor-derived Exosome enrichment and Quantification Assay Kit |
100assays | biological fluids/cell media, Luminometric |
3 外泌体分离/分选产品系列
方法一:简单的一步法分离
产品编号 | 产品名称 | 产品规格 | 样本类型 |
M1001 | ExoPure™ Reagent (Overall Exosome Isolation) | 5T/10T/20T | biological fluids |
M1002 | ExoPure™ Reagent (Overall Exosome Isolation) | 25T/50T | cell media |
M1003 | ExoPure™ Reagent (Overall Exosome Isolation) | 25T/50T | urine |
方法二:Immunobead分选,富集分离外泌体;分离后便于解离,与下游实验兼容;提供0.4um和1.0um两种大小的微球;使用简单、高效、快速。
分选原理:
产品清单:
产品编号 | 产品名称 | 产品规格 | 样本类型 |
M1030 | ExoPure™ 0.4 micron Immunobeads (Overall Exosome Isolation) |
3T/5T/10T/20T | cell media |
M1038 | ExoPure™ 0.4 micron Immunobeads (Overall Exosome Isolation) |
3T/5T/10T/20T | plasma, urine, serum |
M1031 | ExoPure™ 1.0 micron Immunobeads (Overall Exosome Isolation) |
3T/5T/10T/20T | cell media |
M1039 | ExoPure™ 1.0 micron Immunobeads (Overall Exosome Isolation) |
3T/5T/10T/20T | plasma, urine, serum |
M1034 | MouseExoPure™ 0.4 micron Immunobeads (Overall Exosome Isolation) |
3T/5T/10T/20T | mouse biofluids |
M1036 | MouseExoPure™ 0.4 micron Immunobeads (Overall Exosome Isolation) |
3T/5T/10T/20T | mouse cell media |
M1035 | MouseExoPure™ 1.0 micron Immunobeads (Overall Exosome Isolation) |
3T/5T/10T/20T | mouse biofluids |
M1037 | MouseExoPure™ 1.0 micron Immunobeads (Overall Exosome Isolation) |
3T/5T/10T/20T | mouse cell media |
M1032 | TumorExoPure™ 0.4 micron Immunobeads (Tumor-derived Exosome Isolation) |
3T/5T/10T/20T | biofluids |
M1033 | TumorExoPure™ 1.0 micron Immunobeads (Tumor-derived Exosome Isolation) |
3T/5T/10T/20T | biofluids |
方法三:Isolation Kit分选试剂盒
产品清单:
产品编号 | 产品名称 | 产品规格 |
K1241-2 | Exosome Isolation Kit (Bio Fluids) | 2T |
K1237-2 | Exosome Isolation Kit (Cell Media) | 2T |
K1237-10 | Exosome Isolation Kit (Cell Media) | 10T |
K1238-2 | Exosome Isolation Kit (Serum, Plasma) | 2T |
K1238-10 | Exosome Isolation Kit (Serum, Plasma) | 10T |
K1239-10 | Exosome Isolation Kit (Stem Cell Media) | 10T |
K1240-2 | Exosome Isolation Kit (Urine) | 2T |
K1240-10 | Exosome Isolation Kit (Urine) | 10T |
K1241-10 | Exosome Isolation Kit (Bio Fluids) | 10T |
K1239-2 | Exosome Isolation Kit (Stem Cell Media) | 2T |
4 外泌体DNA提取试剂盒Exosome DNA Extraction Kits
产品编号 | 产品名称 | 产品规格 |
K1230 | ExoDNAPS™ Circulating and Exosome-associated DNA extraction kit (Human Plasma/Serum, 20 reactions) |
20T/40T |
K1231 | ExoDNAUC™ Circulating and Exosome-associated DNA extraction kit (Urine/Cell Media, 20 reactions) |
20/40 |
5 外泌体RNA提取试剂盒 Exosome DNA Extraction Kits
产品特点:简单、高效提取外泌体总RNA;提取物适用于下游实验。
产品编号 | 产品名称 | 产品规格 |
K1242 | ExoDNA™ Extraction Kit | 25T/50T |
K1222 | BasicExoRNA™ Basic RNA extraction kit (from pre-isolated exosomes) | 10T/20T/30T |
K1221 | TumorExoRNA™ Tumor-derived Exosome immunocapture and RNA extraction kit | 10T/20T/30T |
6.外泌体标准品
产品特点:用于阳性质控;源于多种样本;经多种实验(FCM,WB,ELISA)反复验证。
7.外泌体荧光标记抗体
产品编号 | 产品名称 | 产品规格 |
A1507-50 | Anti-human Alix Antibody | 100T |
A1506-50 | Anti-human Caveolin-1 antibody | 100T |
A1503-50 | Anti-human CD41 antibody | 100T |
A1505-50 | Anti-human CD44 antibody | 100T |
A1502-50 | Anti-human CD63 antibody | 100T |
A1504-50 | Anti-human CD81 antibody | 100T |
A1500-50 | Anti-human CD9 antibody | 100T |
A1501-50 | Anti-human CD9 biotin conjugated antibody | 100T |
A1510-50 | Anti-human Flotillin antibody | 100T |
A1512-50 | Anti-human HSP70 antibody | 100T |
A1511-50 | Anti-human Rab5 antibody | 100T |
A1513-50 | Anti-human TM9SF3 antibody | 100T |
A1508-50 | Anti-human TM9SF4 antibody | 100T |
A1514-50 | Anti-human TM9SF4 antibody | 100T |
CD41流式检测结果图:
上海优宁维生物科技股份有限公司