首页  >  资源与文章  >  产品资讯  >  7个外泌体解决方案,产品线总攻略

7个外泌体解决方案,产品线总攻略

时间:2016-11-30 01:46:34
浏览次数:18608
分享:

 

外泌体产品线总攻略—小优助手来帮您!

 

本文首发公众平台:流式专家,欢迎订阅

 

外泌体是细胞经过"内吞-融合-外排"等一系列调控过程而形成的细胞外纳米级(直径为30-100nm)小囊泡。外泌体可以携带蛋白,运送RNA,在细胞间物质和信息转导中起重要作用。外泌体可能通过调控免疫功能,促进肿瘤血管新生和肿瘤转移,以及直接作用于肿瘤细胞等途径,影响肿瘤的进展。外泌体由细胞分泌释放出来,在血液等体液内传播,最后又可被其他细胞吞噬,是细胞间通讯的重要介质。

 

 

外泌体目前被视为特异性分泌的膜泡,参与细胞间通讯,对外泌体的研究兴趣日益增长,从外泌体研究方向而言,干细胞、免疫、microRNA、靶向给药等都是热门研究领域,2013年诺贝尔生物/医学奖授予三位在细胞囊泡方面贡献突出的科学家,使外泌体的研究达到全新的高度,外泌体将在癌症的诊断及治疗等领域发挥重要作用。

 
外泌体产生过程的示意图:
 
 
 

 

Biovision公司则是提供了最全的外泌体解决方案:

1 流式检测试剂盒:检测原理如下:

 

产品清单:

 

产品编号 产品名称 产品规格
K1236-20 ExoFACS™ Kit for Cell Culture Media Exosomes 20 reactions
K1232-20 ExoFACS™ Kit for Plasma Exosomes 20 reactions
K1234-20 ExoFACS™ Kit for Saliva Exosomes 20 reactions
K1233-20 ExoFACS™ Kit for Serum Exosomes 20 reactions
K1235-20 ExoFACS™ Kit for Urine Exosomes 20 reactions

 

2.外泌体定量试剂盒:适用不同样本类型;仅需100ul样本;采用夹心法ELISA技术。

产品清单:

 

产品编号 产品名称 产品规格 样本量和显色方法
K1205-100 ExoQuant™ Overall Exosome Capture and Quantification Assay Kit 100assays Cell media, Colorimetri
K1210-100 ExoQuant™ Overall Exosome Capture and Quantification Assay Kit 100assays cell media, Luminometric
K1201-100 ExoQuant™ Overall Exosome Capture and Quantification Assay Kit 100assays human plasma, Colorimetric
K1206-100 ExoQuant™ Overall Exosome Capture and Quantification Assay Kit 100assays human plasma, Luminometric
K1203-100 ExoQuant™ Overall Exosome Capture and Quantification Assay Kit 100assays human serum, Colorimetric
K1208-100 ExoQuant™ Overall Exosome Capture and Quantification Assay Kit 100assays human serum, Luminometric
K1202-100 ExoQuant™ Overall Exosome Capture and Quantification Assay Kit 100assays human urine, Colorimetric
K1207-100 ExoQuant™ Overall Exosome Capture and Quantification Assay Kit 100assays human urine, Luminometric
K1204-100 ExoQuant™ Tumor-derived Exosome enrichment and Quantification
Assay Kit
100assays biological fluids/cell media, Colorimetric
K1209-100 ExoQuant™ Tumor-derived Exosome enrichment and Quantification
Assay Kit
100assays biological fluids/cell media, Luminometric

 

3 外泌体分离/分选产品系列

   方法一:简单的一步法分离

 

 

产品编号 产品名称 产品规格 样本类型
M1001 ExoPure™ Reagent (Overall Exosome Isolation) 5T/10T/20T biological fluids
M1002 ExoPure™ Reagent (Overall Exosome Isolation) 25T/50T cell media
M1003 ExoPure™ Reagent (Overall Exosome Isolation) 25T/50T urine

 

方法二:Immunobead分选,富集分离外泌体;分离后便于解离,与下游实验兼容;提供0.4um和1.0um两种大小的微球;使用简单、高效、快速。

分选原理:

产品清单:

 

产品编号 产品名称 产品规格 样本类型
M1030 ExoPure™ 0.4 micron Immunobeads
(Overall Exosome Isolation)
3T/5T/10T/20T cell media
M1038 ExoPure™ 0.4 micron Immunobeads
(Overall Exosome Isolation)
3T/5T/10T/20T plasma, urine, serum
M1031 ExoPure™ 1.0 micron Immunobeads
(Overall Exosome Isolation)
3T/5T/10T/20T cell media
M1039 ExoPure™ 1.0 micron Immunobeads
(Overall Exosome Isolation)
3T/5T/10T/20T plasma, urine, serum
M1034 MouseExoPure™ 0.4 micron Immunobeads
(Overall Exosome Isolation)
3T/5T/10T/20T mouse biofluids
M1036 MouseExoPure™ 0.4 micron Immunobeads
(Overall Exosome Isolation)
3T/5T/10T/20T mouse cell media
M1035 MouseExoPure™ 1.0 micron Immunobeads
(Overall Exosome Isolation)
3T/5T/10T/20T mouse biofluids
M1037 MouseExoPure™ 1.0 micron Immunobeads
(Overall Exosome Isolation)
3T/5T/10T/20T mouse cell media
M1032 TumorExoPure™ 0.4 micron Immunobeads
(Tumor-derived Exosome Isolation)
3T/5T/10T/20T biofluids
M1033 TumorExoPure™ 1.0 micron Immunobeads
(Tumor-derived Exosome Isolation)
3T/5T/10T/20T biofluids

 

方法三:Isolation Kit分选试剂盒

 

产品清单:

 

产品编号 产品名称 产品规格
K1241-2 Exosome Isolation Kit (Bio Fluids) 2T
K1237-2 Exosome Isolation Kit (Cell Media) 2T
K1237-10 Exosome Isolation Kit (Cell Media) 10T
K1238-2 Exosome Isolation Kit (Serum, Plasma) 2T
K1238-10 Exosome Isolation Kit (Serum, Plasma) 10T
K1239-10 Exosome Isolation Kit (Stem Cell Media) 10T
K1240-2 Exosome Isolation Kit (Urine) 2T
K1240-10 Exosome Isolation Kit (Urine) 10T
K1241-10 Exosome Isolation Kit (Bio Fluids) 10T
K1239-2 Exosome Isolation Kit (Stem Cell Media) 2T

 

 

4 外泌体DNA提取试剂盒Exosome DNA Extraction Kits

 

产品编号 产品名称 产品规格
K1230 ExoDNAPS™ Circulating and Exosome-associated DNA extraction kit
(Human Plasma/Serum, 20 reactions)
20T/40T
K1231 ExoDNAUC™ Circulating and Exosome-associated DNA extraction kit
(Urine/Cell Media, 20 reactions)
20/40

5 外泌体RNA提取试剂盒 Exosome DNA Extraction Kits
产品特点:简单、高效提取外泌体总RNA;提取物适用于下游实验。

 

产品编号 产品名称 产品规格
K1242 ExoDNA™ Extraction Kit 25T/50T
K1222 BasicExoRNA™ Basic RNA extraction kit (from pre-isolated exosomes) 10T/20T/30T
K1221 TumorExoRNA™ Tumor-derived Exosome immunocapture and RNA extraction kit 10T/20T/30T

 

 

6.外泌体标准品
产品特点:用于阳性质控;源于多种样本;经多种实验(FCM,WB,ELISA)反复验证。

 

 

7.外泌体荧光标记抗体


 

产品编号 产品名称 产品规格
A1507-50 Anti-human Alix Antibody 100T
A1506-50 Anti-human Caveolin-1 antibody 100T
A1503-50 Anti-human CD41 antibody 100T
A1505-50 Anti-human CD44 antibody 100T
A1502-50 Anti-human CD63 antibody 100T
A1504-50 Anti-human CD81 antibody 100T
A1500-50 Anti-human CD9 antibody 100T
A1501-50 Anti-human CD9 biotin conjugated antibody 100T
A1510-50 Anti-human Flotillin antibody 100T
A1512-50 Anti-human HSP70 antibody 100T
A1511-50 Anti-human Rab5 antibody 100T
A1513-50 Anti-human TM9SF3 antibody 100T
A1508-50 Anti-human TM9SF4 antibody 100T
 A1514-50 Anti-human TM9SF4 antibody 100T

 

CD41流式检测结果图:

 

 

上海优宁维生物科技股份有限公司