Cytoskeleton® 新年钜惠
小G蛋白热卖研究工具第二件半价!
活动详情:
2. 促销折扣:凡购买清单内任意一件产品,第二件(≤第一件目录价的商品)享受折后再五折优惠!
3. 活动时间:2021年1月9日-2月25日
4. 促销编码:cyto20210109
例:购买A商品第一件10000(目录价)×0.8(常规折扣)=8000(成交价),第二件B商品享受10000(目录价)×0.8(常规折扣)×0.5(第二件折上折)=4000(成交价)。
*注意:享受折后再5折的商品目录价需≤第一件。同时购买多件商品时,目录价较低的一半商品享受折扣。
**活动产品请扫描二维码查看或见底部清单**
小G蛋白(SmG)是一类分子量只有20-30KDa的小蛋白分子,目前发现的小G蛋白包括Rho, Rac, Arf1 & 6, Ras, Ral等。小G蛋白具有GTP酶活性,在多种细胞反应中有开关作用,参与细胞信号通路,也可调节一系列细胞活动,包括分泌活动和细胞骨架的装配等。
Pull-down和G-LISA实验都被广泛地用于研究SmG的活性。Pull-down实验又叫蛋白质体外结合实验,可用于测定活性的GTPase亚型,可作为对GLISA实验的补充。
G-LISA实验是一种改进的ELISA,可测定3-D细胞系、原代细胞裂解液及组织裂解液中有活性的GTPase,使GTPase定量的准确性增加。
以下是分别使用cytoskeleton试剂进行 Pull-down和G-LISA实验得到的结果图。
图1. Pull-down实验结果图。Swiss 3T3细胞血清饥饿24h; 之后,一个样本使用10ng/ml的EGF处理2min(lane 4&5);其他细胞没有经过处理,保持血清饥饿(lane 2&3)。使用Rac1的 pull down实验测定Rac1的活性。使用10μg的PAK-PBD珠子测定500μg的裂解液(lane 2-5)。Lane 1是20ng的重组Rac1-His蛋白,作为WB的标准品。
图2. G-LISA实验结果图。Swiss 3T3细胞分别被Rho激动剂Ⅰ 货号CN01和货号LPA处理,细胞中的RhoA活性随时间变化。通过读取OD490nm的信号值,测定RhoA活性。
Cytoskeleton®专业研发细胞骨架产品,包括Pull-down和G-LISA试剂盒,满足您研究SmG的需求。并且,Cytoskeleton作为研发Pull-down和G-LISA试剂盒的行业内金标准,其产品已被很多客户发表在文献中。
G-LISATMSmall GTPase Activation Assays产品线为小G蛋白激活作用分析及药物筛选提供快速灵敏的研究工具。 产品优势: ● 高灵敏度与准确性 ● 最快3小时得到实验结果 ● 灵活的样本模式 ● 少量的材料需求 ● 多种属适配,可检测小鼠/大鼠/人源组织或细胞 热销靶点: Arf1 Arf6 Cdc42 Rac1,2,3 RalA Ras RhoA
|
|
SiRTM live cell imaging probes产品线独有的细胞膜渗透技术,能在活细胞中对微管蛋白,肌动蛋白,溶酶体,染色体DNA等进行染色。 产品优势: ● 细胞渗透性良好 ● 耐光性良好 ● 超低背景,超高分辨率 ● 远红荧光基团 ● 无细胞毒素 热销产品: SiR-Actin SiR-DNA SiR-Lysosome SiR-Tubulin
|
|
G-switch Activators/Inhibitors产品线是目前用于研究小G蛋白作用(SGPs)的最新技术产品。 产品优势: ● 细胞渗透性良好 ● 快速结果获取 ● 经过多种验证,结果可信度高 ● 广泛文献引用 热销货号: |
产品清单:
小G蛋白活性检测试剂盒 |
||
货号 |
商品名称 |
规格 |
BK001 |
Actin Binding Protein Spin-Down Assay Kit |
KIT 30-100 assays |
BK003 |
Actin Polymerization Biochem Kit |
KIT 30-100 assays |
BK008 |
Ras Activation Assay Biochem Kit |
KIT 50 assays |
BK008-S |
Ras Activation Assay Biochem Kit |
KIT 20 assays |
BK030 |
Combo RhoA/Rac1/Cdc42 Activation Assay Kit |
KIT 3 x 10 assays |
BK032-S |
Arf1 Pulldown Activation Assay Kit |
KIT 20 assays |
BK033-S |
Arf6 Pulldown Activation Assay Kit |
KIT 20 assays |
BK034 |
Cdc42 Pulldown Activation Assay Kit |
KIT 50 assays |
BK034-S |
Cdc42 Pulldown Activation Assay Kit |
KIT 20 assays |
BK035 |
Rac1 Pulldown Activation Assay Kit |
KIT 50 assays |
BK035-S |
Rac1 Pulldown Activation Assay Kit |
KIT 20 assays |
BK036 |
RhoA Pulldown Activation Assay Kit |
KIT 80 assays |
BK036-S |
RhoA Pulldown Activation Assay Kit |
KIT 20 assays |
BK037 |
G-Actin : F-Actin In Vivo Assay Kit |
KIT 30-100 assays |
BK040 |
RalA Activation Assay Biochem Kit |
KIT 50 assays |
BK121 |
RhoA G-LISA Activation Assay (luminescence) |
KIT 96 assays |
BK124 |
RhoA G-LISA Activation Assay (colorimetric) |
KIT 96 assays |
BK124-S |
RhoA G-LISA Activation Assay (colorimetric) |
KIT 24 assays |
BK124-XL |
RhoA G-LISA Activation Assay (colorimetric) |
Large Quantities |
BK125 |
Rac1,2,3 G-LISA Activation Assay Kit (colorimetric) |
KIT 96 assays |
BK126 |
Rac1 G-LISA Activation Assay Kit |
KIT 96 assays |
BK127 |
Cdc42 G-LISA Activation Assay Kit (colorimetric) |
KIT 96 assays |
BK127-S |
Cdc42 G-LISA Activation Assay Kit (colorimetric) |
KIT 24 assays |
BK128 |
Rac1 G-LISA Activation Assay Kit (colorimetric) |
KIT 96 assays |
BK128-S |
Rac1 G-LISA Activation Assay Kit (colorimetric) |
KIT 24 assays |
BK129 |
RalA G-LISA Activation Assay Kit (colorimetric) |
KIT 96 assays |
BK131 |
Ras G-LISA Activation Assay Kit (colorimetric) |
KIT 96 assays |
BK131-XL |
Ras G-LISA Activation Assay Kit |
Large Quantities |
BK132 |
Arf1 G-LISA Activation Assay Kit (colorimetric) |
KIT 96 assays |
BK133 |
Arf6 G-LISA Activation Assay Kit (colorimetric) |
KIT 96 assays |
BK135 |
G-LISA Bundle (BK135 = BK124-S + BK127-S + BK128-S) (colorimetric) |
3 KITS (24 assays/kit) |
BK150 |
RhoA ELISA Kit (colorimetric) |
KIT 96 assays |
小G蛋白激活剂/抑制剂 |
||
货号 |
商品名称 |
规格 |
CN01-A |
Rho Activator |
5 x 10 units |
CN01-B |
Rho Activator |
20 x 10 units |
CN02-A |
Rac and Cdc42 Activator |
5 x 10 units |
CN02-B |
Rac and Cdc42 Activator |
20 x 10 units |
CN03-A |
Rho activator II |
3 x 20 µg |
CN03-B |
Rho activator II |
9 x 20 µg |
CN03-XL |
Rho activator II |
Large Quantities |
CN04-A |
Rho/Rac/Cdc42 Activator I |
3 x 20 µg |
CN04-B |
Rho/Rac/Cdc42 Activator I |
9 x 20 µg |
CT03-A |
C3 transferase protein |
1 x 25 µg |
CT03-C |
C3 transferase protein |
4 x 25 µg |
CT04-A |
Cell Permeable Rho Inhibitor (C3 Trans based)) |
1 x 20 µg |
CT04-B |
Cell Permeable Rho Inhibitor (C3 Trans based)) |
5 x 20 µg |
CT04-C |
Cell Permeable Rho Inhibitor (C3 Trans based)) |
20 x 20 µg |
骨架蛋白染料 |
||
货号 |
商品名称 |
规格 |
CY-SC001 |
SiR-Actin Kit |
50-300 slides |
CY-SC002 |
SiR-Tubulin Kit |
50-300 slides |
CY-SC006 |
Cytoskeleton Kit (SiR-Actin + SiR-Tubulin) |
50-300 slides each |
CY-SC007 |
SiR-DNA Kit |
50-300 slides |
CY-SC012 |
SiR-Lysosome Kit |
50-300 slides |
CY-SC013 |
SiR700-Actin Kit |
35-200 slides |
CY-SC014 |
SiR700-Tubulin Kit |
35-200 slides |
CY-SC015 |
SiR700-DNA Kit |
35-200 slides |
CY-SC016 |
SiR700-Lysosome Kit |
35-200 slides |
CY-SC020 |
Flipper TR membrane tension sensor |
35-200 slides |
CY-SC101 |
SPY505-DNA Probe |
100 slides |
CY-SC201 |
SPY555-DNA Probe |
100 slides |
CY-SC202 |
SPY555-Actin Probe |
100 slides |
CY-SC203 |
SPY555-Tubulin Probe |
100 slides |
CY-SC204 |
SPY555-BG Substrate, Abs./Em. 555/580 nm |
35 nmoles |
CY-SC301 |
SPY595-DNA Probe |
100 slides |
CY-SC401 |
SPY620-DNA Probe |
100 slides |
CY-SC402 |
SPY620-Actin Probe |
100 slides |
CY-SC404 |
SPY620-BG Substrate, Abs./Em. 619/635 nm |
35 nmoles |
CY-SC501 |
SPY650-DNA Probe |
100 slides |
CY-SC503 |
SPY650-Tubulin Probe |
100 slides |
CY-SC504 |
SiR650-BG Substrate, Abs./Em. 652/674 nm |
35 nmoles |
CY-SC601 |
SPY700-DNA Probe |
100 slides |
CY-SC604 |
SiR700-BG Substrate, Abs./Em. 696/718 nm |
35 nmoles |
细胞膜荧光探针 |
||
货号 |
商品名称 |
规格 |
MG01-02 |
MemGlow 488 - Fluorogenic Plasma Membrane probe ex 499 em 507 50-300 slides (MEMBRIGHT Family Probe) |
50-300 slides |
MG01-10 |
MemGlow 488 - Fluorogenic Plasma Membrane probe ex 499 em 507 250-1500 slides (MEMBRIGHT Family Probe) |
250-1500 slides |
MG02-02 |
MemGlow 560 - Fluorogenic Plasma Membrane probe ex 555 em 570 50-300 slides (MEMBRIGHT Family Probe) |
50-300 slides |
MG02-10 |
MemGlow 560 - Fluorogenic Plasma Membrane probe ex 555 em 570 250-1500 slides (MEMBRIGHT Family Probe) |
250-1500 slides |
MG03-02 |
MemGlow 590 - Fluorogenic Plasma Membrane probe ex 580 em 620 50-300 slides (MEMBRIGHT Family Probe) |
50-300 slides |
MG03-10 |
MemGlow 590 - Fluorogenic Plasma Membrane probe ex 580 em 620 250-1500 slides (MEMBRIGHT Family Probe) |
250-1500 slides |
MG04-02 |
MemGlow 640 - Fluorogenic Plasma Membrane probe ex 650 em 673 50-300 slides (MEMBRIGHT Family Probe) |
50-300 slides |
MG04-10 |
MemGlow 640 - Fluorogenic Plasma Membrane probe ex 650 em 673 250-1500 slides (MEMBRIGHT Family Probe) |
250-1500 slides |
MG05-02 |
MemGlow 700 - Fluorogenic Plasma Membrane probe ex 650 em 720 50-300 slides (MEMBRIGHT Family Probe) |
50-300 slides |
MG05-10 |
MemGlow 700 - Fluorogenic Plasma Membrane probe ex 650 em 720 250-1500 slides (MEMBRIGHT Family Probe) |
250-1500 slides |
抗体 |
||
货号 |
商品名称 |
规格 |
AAN02 |
Anti-Pan Actin Mouse Monoclonal Antibody (Clone 7A8.2.1) |
1 x 500 ul |
AAN02-S |
Anti-Pan Actin Mouse Monoclonal Antibody (Clone 7A8.2.1) |
1 x 125 ul |
更多详情请咨询小优~
上海优宁维生物科技股份有限公司