BioXcell特色推荐——InVivoMAb anti-mouse IL-4

一产品背景

IL-4主要由活化的Th2细胞和自然杀伤细胞表达，较低水平由肥大细胞和嗜碱性粒细胞表达。IL-4通过IL-4Rα发出信号。当IL-4与受体结合时，IL-4刺激活化的B和T淋巴细胞增殖，以及B细胞向浆细胞的分化。它还诱导B细胞向IgE类转换，上调MHC II类的产生，同时减少Th1细胞、巨噬细胞、IFNγ和树突状细胞IL-12的产生。像其他Th2相关细胞因子一样，IL-4参与了过敏性哮喘患者肺部观察到的气道炎症。克隆号11B11单克隆抗体已被证明能中和天然或重组IL-4的生物活性。

二抗体的应用 Application& 产品信息

产品货号	BE0045
产品规格	1/5/25/50/100mg
抗体亚型	Rat IgG1, κ
推荐同型对照	InVivoMAb rat IgG1 isotype control, anti-horseradish peroxidase（货号：BE0088）
推荐抗体稀释液	InVivoPure™ pH 7.0 Dilution Buffer（货号：IP0070）
免疫原	Partially purified native mouse IL-4
应用	in vivo IL-4 neutralization、in vitro IL-4 neutralization,in vivo IL-4 receptor stimulation (as a complex with IL-4),Flow cytometry、Western blot
产品形式	PBS, pH 7.0,Contains no stabilizers or preservatives
内毒素水平	<2EU/mg (<0.002EU/μg)
纯度	>95% Determined by SDS-PAGE
无菌处理	0.2 μM filtered
生产形式	从组织培养上清液中纯化得到。
纯化形式	Protein G
RRID	AB_1107707
分子量大小	150 kDa
保存条件	抗体原溶液应保存在4°C条件下，不要冷冻保存。

三产品已发表文献

用途	已发表文献文章
in vivo IL-4 neutralization &in vivo IL-4 receptor stimulation (as a complex with IL-4)	Gaya, M., et al. (2018). “Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells.” Cell 172(3): 517-533 e520
in vitro IL-4 neutralization	Clever, D., et al. (2016). “Oxygen Sensing by T Cells Establishes an Immunologically Tolerant Metastatic Niche.” Cell 166(5): 1117-1131 e1114.
in vitro IL-4 neutralization	Choi, Y. S., et al. (2015). “LEF-1 and TCF-1 orchestrate TFH differentiation by regulating differentiation circuits upstream of the transcriptional repressor Bcl6.” Nat Immunol 16(9): 980-990
in vitro IL-4 neutralization &Flow cytometry	Gu, A. D., et al. (2015). “A critical role for transcription factor Smad4 in T cell function that is independent of transforming growth factor beta receptor signaling.” Immunity 42(1): 68-79
in vitro IL-4 neutralization	Hill, E. V., et al. (2015). “Glycogen synthase kinase-3 controls IL-10 expression in CD4(+) effector T-cell subsets through epigenetic modification of the IL-10 promoter.” Eur J Immunol 45(4): 1103-1115
in vitro IL-4 neutralization	Hou, L., et al. (2015). “The protease cathepsin L regulates Th17 cell differentiation.” J Autoimmun. S0896-8411(15): 30024-X
in vitro IL-4 neutralization	Kim, Y. U., et al. (2015). “Regulation of autoimmune germinal center reactions in lupus-prone BXD2 mice by follicular helper T cells.” PLoS One 10(3): e0120294
in vitro IL-4 neutralization	Tang, W., et al. (2014). “The oncoprotein and transcriptional regulator Bcl-3 governs plasticity and pathogenicity of autoimmune T cells.” Immunity 41(4): 555-566

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