BioXcell特色产品推荐之：InVivoMAb anti-human CD4

一产品介绍

CD4抗原是一种55 kDa的细胞表面I型膜糖蛋白，属于免疫球蛋白超家族。CD4作为协同受体，与T细胞受体(TCR)协同作用，与抗原呈递细胞(APC)展示的ⅱ类MHC分子相互作用。CD4由大多数胸腺细胞和辅助性T细胞(NK-T细胞的一个亚群)表达，并且由树突细胞和巨噬细胞微弱地表达。CD4在T细胞的发育中起着重要作用，是成熟T细胞发挥最佳功能所必需的。据报道，RPA-T4抗体结合CD4的D1结构域，并且不阻断OKT-4抗体的结合。此外，RPA-T4已被证明能阻断HIV gp120蛋白与CD4的结合，并在体外抑制CD4 T细胞的活化。

二产品详情

产品货号	BE0288
产品规格	1/5/25/50/100mg
抗体亚型	Mouse IgG1, κ
推荐同型对照	InVivoMAb mouse IgG1 isotype control, unknown specificity（货号：BE0083）
推荐抗体稀释液	InVivoPure™ pH 7.0 Dilution Buffer（货号：IP0070）
免疫原	Not available or unknown
应用	in vitro CD4 blockade、in vitro blocking of CD4+ T cell activation Immunofluorescence、Immunohistochemistry (frozen)、Flow cytometry
产品形式	PBS , pH 7.0 Contains no stabilizers or preservatives
内毒素水平	<2EU/mg (<0.002EU/μg) 使用 LAL gel clotting 测定
纯度	>95% Determined by SDS-PAGE
无菌处理	0.2 μM filtered
生产形式	从组织培养上清液中纯化得到。
纯化形式	Protein G
RRID	AB_2687811
分子量大小	150 kDa
保存条件	抗体原溶液应保存在4°C条件下，不要冷冻保存。

三已发表文献

用途	已发表文献
Immunofluorescence	Moolla, N., et al. (2016). “Thioredoxin (Trx1) regulates CD4 membrane domain localization and is required for efficient CD4-dependent HIV-1 entry.” Biochim Biophys Acta 1860(9): 1854-1863
in vitro blocking of T cell activation	Mayer, C. T., et al. (2013). “CD4 blockade directly inhibits mouse and human CD4(+) T cell functions independent of Foxp3(+) Tregs.” J Autoimmun 47: 73-82
in vitro CD4 blockade	Toma, J., et al. (2011). “Loss of asparagine-linked glycosylation sites in variable region 5 of human immunodeficiency virus type 1 envelope is associated with resistance to CD4 antibody ibalizumab.” J Virol 85(8): 3872-3880
Immunohistochemistry (frozen)	Mack, C. L., et al. (2004). “Biliary atresia is associated with CD4+ Th1 cell-mediated portal tract inflammation.” Pediatr Res 56(1): 79-87.
in vitro CD4 blockade	Geijtenbeek, T. B., et al. (2000). “DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells.” Cell 100(5): 587-597
in vitro CD4 blockade	Moir, S., et al. (1999). “CD40-Mediated induction of CD4 and CXCR4 on B lymphocytes correlates with restricted susceptibility to human immunodeficiency virus type 1 infection: potential role of B lymphocytes as a viral reservoir.” J Virol 73(10): 7972-7980

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