BioXcell特色产品推荐之：InVivoMAb anti-mouse CD47 (IAP)

一、产品介绍
CD47是一种约50 kDa的糖基化五跨膜蛋白，由造血细胞如T和B淋巴细胞、单核细胞、血小板和红细胞以及非造血细胞普遍表达。CD47参与一系列细胞过程，包括凋亡、增殖、粘附和迁移。此外，它在免疫和血管生成反应中起关键作用。CD47是血小板反应素-1 (TSP-1)的受体，TSP-1是一种分泌型糖蛋白，在血管发育和血管生成中起作用。已经发现CD47在许多不同的肿瘤细胞中过度表达。正因为如此，抗CD47单克隆抗体被提出并研究作为人类癌症的治疗方法。已经证明克隆号MIAP301抗体在体内和体外能够中和CD47。

二、产品详情

产品详情
产品货号	BE0270
产品规格	1/5/25/50/100mg
抗体亚型	Rat IgG2a, κ
推荐同型对照	InVivoMAb rat IgG2a isotype control, anti-trinitrophenol（货号：BE0089）
推荐抗体稀释液	InVivoPure™ pH 7.0 Dilution Buffer（货号：IP0070）
免疫原	Intact CD47 purified from placenta
应用	in vivo CD47 blockade、in vitro CD47 blockade、Immunofluorescence
产品形式	PBS , pH 7.0 Contains no stabilizers or preservatives
内毒素水平	<2EU/mg (<0.002EU/μg) 使用 LAL gel clotting 测定
纯度	>95% Determined by SDS-PAGE
无菌处理	0.2 μM filtered
生产形式	从组织培养上清液中纯化得到。
纯化形式	Protein G
RRID	AB_2687793
分子量大小	150 kDa
保存条件	抗体原溶液应保存在4°C条件下，不要冷冻保存。

三、已发表文献

用途	已发表文献
in vitro CD47 blockade	Liu, M., et al. (2019). “Metabolic rewiring of macrophages by CpG potentiates clearance of cancer cells and overcomes tumor-expressed CD47-mediated ‘don’t-eat-me’ signal.” Nat Immunol 20(3): 265-275.
in vivo CD47 blockade	Pan, Y., et al. (2019). “Single-cell RNA sequencing reveals compartmental remodeling of tumor-infiltrating immune cells induced by anti-CD47 targeting in pancreatic cancer.” J Hematol Oncol 12(1): 124.
in vivo CD47 blockade	Reed, M., et al. (2019). “Epithelial CD47 is critical for mucosal repair in the murine intestine in vivo.” Nat Commun 10(1): 5004
in vivo CD47 blockade	Sallets, A., et al. (2018). “Enhancing immunotherapy of STING agonist for lymphoma in preclinical models.” Blood Adv 2(17): 2230-2241
in vivo CD47 blockade	Wu, L., et al. (2018). “Anti-CD47 treatment enhances anti-tumor T-cell immunity and improves immunosuppressive environment in head and neck squamous cell carcinoma.” Oncoimmunology 7(4): e1397248
in vivo CD47 blockade	Xu, M. M., et al. (2017). “Dendritic Cells but Not Macrophages Sense Tumor Mitochondrial DNA for Cross-priming through Signal Regulatory Protein alpha Signaling.” Immunity 47(2): 363-373 e365
in vitro CD47 blockade	Hsieh, C. P., et al. (2015). “Deficits in cerebellar granule cell development and social interactions in CD47 knockout mice.” Dev Neurobiol 75(5): 463-484
in vivo CD47 blockade	Liu, X., et al. (2015). “CD47 blockade triggers T cell-mediated destruction of immunogenic tumors.” Nat Med 21(10): 1209-1215
in vivo CD47 blockade	Maute, R. L., et al. (2015). “Engineering high-affinity PD-1 variants for optimized immunotherapy and immuno-PET imaging.” Proc Natl Acad Sci U S A 112(47): E6506-6514
in vivo CD47 blockade	Shi, L., et al. (2015). “CD47 deficiency ameliorates autoimmune nephritis in Fas(lpr) mice by suppressing IgG autoantibody production.” J Pathol 237(3): 285-295
in vitro CD47 blockade	Vermeer, D. W., et al. (2013). “Radiation-induced loss of cell surface CD47 enhances immune-mediated clearance of human papillomavirus-positive cancer.” Int J Cancer 133(1): 120-129
in vitro CD47 blockade	Majeti, R., et al. (2009). “CD47 is an adverse prognostic factor and therapeutic antibody target on human acute myeloid leukemia stem cells.” Cell 138(2): 286-299
in vivo CD47 blockade	Chang, H. P., et al. (2001). “Functional blocking of integrin-associated protein impairs memory retention and decreases glutamate release from the hippocampus.” Neuroscience 102(2): 289-296

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