使用MSD111因子试剂盒，汇报在哪里

实体瘤是由癌细胞和宿主间质细胞组成的器官，由血管系统滋养，并嵌入细胞外基质中。这些细胞、周围基质和局部细胞微环境之间的相互作用影响某些基因的表达，这些基因的产物控制肿瘤的病理生理特征，控制肿瘤的进展，并影响肿瘤对各种疗法的反应。

在过去的几年里，免疫疗法改变了我们治疗实体瘤的方式，包括黑色素瘤、肺癌、头颈癌、乳腺癌、肾癌和膀胱癌。许多癌症患者已经从免疫疗法中获益，一些转移性疾病的治疗已经成为可能。在不久的将来，免疫治疗药物以及与其他药物联合使用的额外批准也在预料之中。

实体瘤免疫检查点的筛选，免疫治疗的现有和新兴生物标志物的研究及发现至关重要。美国MSD（Meso Scale Discovery）公司现新上市一款肿瘤免疫新品试剂盒，在原有的71和87多因子组合检测试剂盒的基础上升级，推出111因子肿瘤免疫新品试剂盒！其中包括炎症因子、代谢指标、肿瘤免疫相关指标等，在医药研发，临床研究，细胞治疗等领域应用广泛。

新品上市，活动自然少不了

MSD携手LabEX联合拼单大促，每份样本只要300ul，同时检测111个指标，仅需4388元，接受1-3人组合预定，包含标准曲线及预实验！
数量有限，预定从速！

高分文献分享

文章：Luo H, Wang N, Huang L, et al. Inflammatory signatures for quick diagnosis of life-threatening infection during the CAR T-cell therapy. J Immunother Cancer. 2019;7(1):271. Published 2019 Oct 22. doi:10.1186/s40425-019-0767-x

作者单位：华中科技大学同济医学院

使用试剂盒：

细胞因子检测检测方法&相关发现：A 70-biomarker panel (Meso Scale Discovery, Germany, Cat. K1508K) mainly containing various inflammatory factors was employed in discovery and training phases according to instructions (70 biomarkers were listed in Additional file 1: Figure S4). Relative changes (versus healthy donors) were calculated and log transformation was done before analysis. The MannWhitney unpaired test was used for the inter-group comparison. A stepwise logistic regression was utilized for making prediction model. For the detection of IL-8,IFN-γ and IL-1β in the prediction model, a new panel containing the three cytokines was designed and used for validation. Hosmer-lemeshow test and receiver operating characteristic (ROC) curve were used to evaluate the prediction model.

文章：Locke, Frederick L.,et al. "Phase 1 Results of ZUMA-1: A Multicenter Study of KTE-C19Anti-CD19 CAR T Cell Therapy in Refractory Aggressive Lymphoma." MolecularTherapy the Journal of the American Society of Gene Therapy 25.1(2017):285.

作者单位：Kite制药有限公司，美国MD安德森癌症中心，美国Moffitt癌症中心

使用试剂盒：

细胞因子检测检测方法&相关发现：Samples were analyzed using thefollowing MSD kits: MSD V-PLEX Plus Angiogenesis Panel 1 (Human) Kit (bFGF,Flt-1/VEGFR-1, PlGF, Tie-2, VEGF-A, VEGF-C, VEGF-D),MSDV-PLEX Plus ChemokinePanel 1(Human)Kit (Eotaxin, Eotaxin-3, IL-8 [HA], IP-10, MCP-1, MCP-4, MDC, MIP-1a, MIP-1b,TARC),MSDV-PLEX Plus Cytokine Panel 1 (Human) Kit (GM-CSF, IL-12/IL-23p40,IL-15, IL-16, IL-17A, IL-1a, IL-5, IL-7, TNF-b), MSDV-PLEX Plus ProinflammatoryPanel 1 (Human) Kit (IFN-g, IL-10, IL-12p70, IL-13, IL-1b, IL-2, IL-4, IL-6,IL-8,TNF-a), MSD V-PLEX Plus Vascular Injury Panel 2 (Human) Kit (CRP, ICAM-1,SAA, VCAM-1), All MSD assays were read using a MESO QuickPlex SQ 120 andanalysis was performed using DISCOVERY WORKBENCH 4.0 (MSD).

文章：Asi, Andreasson, et al. "The role ofinterleukin-1β as a predictive biomarker and potential therapeutic targetduring clinical ex vivo lung perfusion." Journal of Heart &Lung Transplantation the Official Publication of the International Society forHeart Transplantation (2017).

单位：纽卡斯尔大学（细胞医学研究所），英国

样本：灌流液和组织裂解物

使用试剂盒：

细胞因子检测检测方法&相关发现：Perfusate and tissue lysates were analyzed with a V-PLEX Human Biomarker 40-Plex Kit and a Human MMP 3-Plex Ultra-SensitiveKit (both Meso Scale Diagnostics, LLC, Rockville, MD). BAL was analyzed using a V-PLEXhuman pro-inflammatory Panel 1 Kit and a Human MMP3-Plex Ultra-Sensitive Kit (both Meso ScaleDiagnostics, LLC).

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