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Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Simple Western™ | 1:10 - 1:50 |
Immunoprecipitation | 1:50 |




Specificity/Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey






参考图片
Western blot analysis of extracts from 293 (human), COS (monkey), L929 (mouse) and PC12 (rat) cells, using Bax Antibody.
Western blot 免疫印迹。用Bax Antibody研究 293 (人源)、COS (猴子来源)、L929 (小鼠源) 和 PC12 (大鼠) 细胞的细胞提取液。
Immunoprecipitation of Bax from Jurkat cell extract, using Bax Antibody. Lane 1 is the lysate control; lane 2 is antibody alone; lane 3 is antibody plus lysate.
用Bax Antibody经免疫沉淀分析Jurkat细胞提取液中的Bax,泳道1 是裂解液的对照;泳道2是只有抗体;泳道3是抗体加上裂解液。
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-), SignalSilence® Bax siRNA I (+) or SignalSilence® Bax siRNA II (+), using Bax Antibody and α-Tubulin (11H10) Rabbit mAb #2125. The Bax Antibody confirms silencing of Bax expression and α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of Bax siRNA.
Western blot免疫印迹分析转染了100 nM SignalSilence® Control siRNA (荧光偶联) #6201 (-),SignalSilence® Bax siRNA I (+) 或SignalSilence® Bax siRNA II(+)的HeLa细胞的细胞提取液,所用抗体为Bax Antibody 和α-Tubulin (11H10) Rabbit mAb 兔单抗#2125。Bax Antibody证实对Bax表达的沉默,α-Tubulin (11H10) Rabbit mAb 兔单抗作为上样量的对照,并验证Bax siRNA的特异性。