Bax Antibody
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Bax Antibody

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品牌: CST
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    分子量:
    20
    20
    反应种属:
    Human,Mouse,Rat,Monkey,
    Human,Mouse,Rat,Monkey,
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    产品介绍
    产品信息
    抗原名称
    Bax
    来源纯化
    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal residues of human Bax. Antibodies are purified by protein A and peptide affinity chromatography.
    宿主
    Rabbit
    简单描述
    Polyclonal Antibody for studying Bax. Cited in 1999 publications. Validated for WB, WB, IP. Available in 2 sizes. Highly specific and rigorously validated in-house, Bax Antibody (CST #2772) is ready to ship.
    商品描述

    Product Usage Information

    ApplicationDilution
    Western Blotting1:1000
    Simple Western™1:10 - 1:50
    Immunoprecipitation1:50
    分子量
    20
    研究领域
    癌症,细胞生物学,纤维化,神经科学,
    应用
    反应种属
    Human,Mouse,Rat,Monkey,
    目标/特异性

    Specificity/Sensitivity

    Bax Antibody detects endogenous levels of total Bax protein. The antibody does not cross-react with other Bcl-2 family members.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    敏感性
    Endogenous
    背景
    背景
    Bax 是因线粒体应激引起的细胞凋亡的关键组分 (1)。受凋亡性刺激后,Bax 形成寡聚体并从细胞溶胶转位至线粒体膜 (2)。通过与线粒体膜上的孔蛋白相互作用,Bax 增加膜的通透性,这导致细胞色素 C 从线粒体释放、激活 caspase-9 和 caspase 激活通路启动以凋亡 (3,4)。 Wei, M.C. et al. (2001) Science 292, 727-30. Jürgensmeier, J.M. et al. (1998) Proc Natl Acad Sci U S A 95, 4997-5002. Narita, M. et al. (1998) Proc Natl Acad Sci U S A 95, 14681-6. Marzo, I. et al. (1998) Science 281, 2027-31.
    研究领域
    癌症,细胞生物学,纤维化,神经科学,
    翻译后修饰
    Unmodified
    制备和贮存
    保存方式
    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
    数据库链接
    Entrez-Gene ID
    581
    UniProt ID
    Q07812

    参考图片

    Western blot analysis of extracts from 293 (human), COS (monkey), L929 (mouse) and PC12 (rat) cells, using Bax Antibody.

    Western blot 免疫印迹。用Bax Antibody研究 293 (人源)、COS (猴子来源)、L929 (小鼠源) 和 PC12 (大鼠) 细胞的细胞提取液。

    Immunoprecipitation of Bax from Jurkat cell extract, using Bax Antibody. Lane 1 is the lysate control; lane 2 is antibody alone; lane 3 is antibody plus lysate.

    用Bax Antibody经免疫沉淀分析Jurkat细胞提取液中的Bax,泳道1 是裂解液的对照;泳道2是只有抗体;泳道3是抗体加上裂解液。

    Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-), SignalSilence® Bax siRNA I (+) or SignalSilence® Bax siRNA II (+), using Bax Antibody and α-Tubulin (11H10) Rabbit mAb #2125. The Bax Antibody confirms silencing of Bax expression and α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of Bax siRNA.

    Western blot免疫印迹分析转染了100 nM SignalSilence® Control siRNA (荧光偶联) #6201 (-),SignalSilence® Bax siRNA I (+) 或SignalSilence® Bax siRNA II(+)的HeLa细胞的细胞提取液,所用抗体为Bax Antibody 和α-Tubulin (11H10) Rabbit mAb 兔单抗#2125。Bax Antibody证实对Bax表达的沉默,α-Tubulin (11H10) Rabbit mAb 兔单抗作为上样量的对照,并验证Bax siRNA的特异性。

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    货号:
    2772S
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