Rabbit anti-β-Actin Polyclonal Antibody

1/2

Rabbit anti-β-Actin Polyclonal Antibody

品牌： Absin

下载产品说明书

用小程序，查商品更便捷

对比

咨询

参数

分子量：

Predicted molecular weight: 42kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

反应种属：

Human;Mouse;Rat;Pig;Zebrafish;Dog;Monkey;Fish

展开

产品介绍

产品信息

耦联标记

Unlabeled

纯化方式

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.

宿主

Rabbit

免疫原

A synthesized peptide derived from human Beta actin.

商品描述

Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility.

分子量

克隆类别

Polyclonal Antibody

浓度

1mg/ml

应用

实验应用

WB: 1:3000-1:20000, IHC: 1:200, IF: 1:100-1:500, ICC: 1:100-1:500, ELISA(peptide): 1:20000-1:40000

反应种属

Human;Mouse;Rat;Pig;Zebrafish;Dog;Monkey;Fish

目标/特异性

Beta actin antibody detects endogenous levels of total Beta actin.

背景

别名

β-Actin抗体

翻译后修饰

Unmodified

细胞定位

Cytoskeleton;Cytosol;Extracellular region or secreted;Nucleus;Plasma Membrane;

制备和贮存

存储溶液

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

保存方式

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles

参考图片

Western blot analysis of extracts from various samples, using beta Actin Antibody. Lane 1: Rat kidney, blocked with antigen-specific peptides. Lane 2: Rat kidney. Lane 3: Mouse kidney. Lane 4: Hepg2 cells(serum starvation treatment). Lane 5: Hela cells(heat-shock treatment).

abs132001 staining HEPG2 cells by ICC/IF. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600 was used as secondary antibody.

声明：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。

货号：

abs132001-50ug

一键复制

询价

优宁维现货

50ug

100ug

选择数量

当前规格1件起购

配送至

预计2-5个工作日送达，快递: 免运费，若需干冰额外收费

询价大包装询价