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品牌: CST
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分子量:
92
92
反应种属:
Human,Mouse,Rat,Monkey,
Human,Mouse,Rat,Monkey,
产品介绍
产品信息
抗原名称
beta-Catenin
![](/4f36f9ee8fdd57778382495a16d6667c.png)
来源纯化
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues around Ser37 of human β-catenin. Antibodies are purified by protein A and peptide affinity chromatography.
![](/4f36f9ee8fdd57778382495a16d6667c.png)
宿主
Rabbit
![](/4f36f9ee8fdd57778382495a16d6667c.png)
简单描述
Polyclonal Antibody for studying Catenin-beta. Cited in 675 publications. Validated for WB, IP, IHC. Available in 2 sizes. Highly specific and rigorously validated in-house, β-Catenin Antibody (CST #9562) is ready to ship.
![](/4f36f9ee8fdd57778382495a16d6667c.png)
商品描述
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:100 |
Immunohistochemistry (Paraffin) | 1:400 |
![](/4f36f9ee8fdd57778382495a16d6667c.png)
分子量
92
![](/4f36f9ee8fdd57778382495a16d6667c.png)
内毒素水平
斑马鱼 , 猪
![](/4f36f9ee8fdd57778382495a16d6667c.png)
研究领域
癌症,发育生物学与干细胞研究,纤维化,神经科学,
![](/4f36f9ee8fdd57778382495a16d6667c.png)
应用
反应种属
Human,Mouse,Rat,Monkey,
![](/4f36f9ee8fdd57778382495a16d6667c.png)
目标/特异性
Specificity/Sensitivity
β-Catenin Antibody detects endogenous levels total of β-catenin. This antibody does cross-react with endogenous levels of γ-catenin.
Species Reactivity:
Human, Mouse, Rat, Monkey
![](/4f36f9ee8fdd57778382495a16d6667c.png)
敏感性
Endogenous
![](/4f36f9ee8fdd57778382495a16d6667c.png)
背景
背景
β-Catenin是 Wnt 信号转导通路中的一个关键下游效应分子 (1)。它与脊椎动物的两个主要生物学过程有关:早期胚胎发育(2)和肿瘤发生(3)。CK1 可以在 Ser45 位点使 β-Catenin 磷酸化。该磷酸化会导致 β-Catenin 随后能被 GSK-3β 磷酸化 (4-6)。GSK-3β 通过在 Ser33、Ser37 和 Thr41 位点磷酸化 β-Catenin以将其降解 (7)。这些位点发生突变会提高 β-Catenin 的稳定性,并且已在多种肿瘤细胞系中发现了这些突变 (8)。
Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-3305.
Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88.
Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21.
Amit, S. et al. (2002) Genes Dev 16, 1066-76.
Liu, C. et al. (2002) Cell 108, 837-47.
Yanagawa, S. et al. (2002) EMBO J 21, 1733-42.
Yost, C. et al. (1996) Genes Dev 10, 1443-54.
Morin, P.J. et al. (1997) Science 275, 1787-90.
![](/4f36f9ee8fdd57778382495a16d6667c.png)
研究领域
癌症,发育生物学与干细胞研究,纤维化,神经科学,
翻译后修饰
Unmodified
![](/4f36f9ee8fdd57778382495a16d6667c.png)
制备和贮存
保存方式
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
数据库链接
Entrez-Gene ID
1499
![](/4f36f9ee8fdd57778382495a16d6667c.png)
UniProt ID
P35222
![](/4f36f9ee8fdd57778382495a16d6667c.png)
参考图片
Western blot analysis of extracts from 293, HeLa, NIH/3T3 and C6 cells using β-Catenin Antibody.
使用 β-Catenin抗体对293,HeLa,NIH/3T3和C6细胞提取物进行western blot分析。
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using beta-Catenin Antibody.
使用beta-Catenin抗体对石蜡包埋的人乳腺癌组织进行免疫组化分析。
Western blot analysis of extracts from SW480 cells using β-Catenin Antibody.
使用β-Catenin抗体对SW480细胞提取物进行western blot分析。
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