BIO

GSK-3

356.17

DMSO ：71 mg/mL (199.34 mM)
Ethanol：21 mg/mL (58.96 mM)

red brown to purple solid

BIO is a specific inhibitor of glycogen synthase kinase-3 (GSK-3), with IC50 of 5 nM for GSK-3α/β, shows > 16-fold selectivity over CDK5. BIO interacts within the ATP binding pocket of these kinases, reduces β-catenin phosphorylation on a GSK-3-specific site in cellular models, closely mimicks Wnt signaling in Xenopus embryos. In human and mouse embryonic stem cells, BIO maintains the undifferentiated phenotype and sustains expression of the pluripotent state-specific transcription factors Oct-3/4, Rex-1 and Nanog. BIO-mediated Wnt activation is functionally reversible, as withdrawal of the compound leads to normal multidifferentiation programs in both human and mouse embryonic stem cells. BIO promotes proliferation in mammalian cardiomyocytes. 6BIO is also a pan-JAK inhibitor, with IC50 values of 0.03, 1.5, 8.0, 0.5 μM for TYK2, JAK1, JAK2 and JAK3, respectively. BIO selectively inhibits phosphorylation of STAT3 and induces apoptosis of human melanoma cells.

BIO (50 mg/kg, p.o.) suppresses melanoma tumor growth in a mouse xenograft model.

6BIO; 6-BIO; GSK 3 IX; GSK 3 Inhibitor IX; MLS 2052; MLS2052; GSK-3 Inhibitor IX, 6-bromoindirubin-3-oxime

C₁₆H₁₀BrN₃O₂

667463-62-9