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Anti-biotin (D5A7) Rabbit mAb (HRP Conjugate)

品牌： CST

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参数

反应种属：

All

来源宿主：

Rabbit

产品介绍

产品信息

耦联标记

HRP

抗原名称

biotin

来源纯化

Monoclonal antibody is produced by immunizing animals with a biotinylated protein.

宿主

Rabbit

简单描述

Secondary Antibody for studying Biotin (for Secondary Abs) in the research area.

商品描述

Product Usage Information

Recommended Antibody Dilutions:ELISA-peptide 1:1000

应用

反应种属

All

目标/特异性

Specificity/Sensitivity

Anti-biotin (D5A7) Rabbit mAb (HRP Conjugate) recognizes biotin attached to proteins, peptides, oligonucleotides and solid matrices.

Species Reactivity:

Rabbit

背景

Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 2 mg/ml bovine serum albumin (BSA), and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

参考图片

Western blot analysis of extracts from HeLa cells, untreated or treated with Staurosporine #9953 (1 μM, 3 hr) using Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb (Biotinylated) primary antibody and Anti-Biotin (D5A7) Rabbit mAb (HRP Conjugate) as secondary detection antibody.Western blot分析HeLa细胞提取物，未刺激组或采用Staurosporine #9953 (1 μM, 3 hr) 刺激组，所用抗体为 Cleaved ARP (Asp214) (D64E10) XP ® Rabbit mAb (Biotinylated) 作为一抗and Anti-Biotin (D5A7) Rabbit mAb (HRP Conjugate) 作为二抗检测抗体。

Chemiluminescent detection of phosphorylated and non-phosphorylated Met peptides with #4033 Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb (Biotinylated) in conjunction with #5571 Anti-biotin (D5A7) Rabbit mAb (HRP Conjugate).化学发光分析磷酸化和未发生磷酸化的Met肽段，所用抗体为#4033 Phospho-Met (Tyr1234/1235) (D26) XP ® Rabbit mAb (生物素化) 联合#5571 Anti-biotin (D5A7) Rabbit mAb (HRP 标记).

Relative fluorescence of biotinylated and non-biotinylated peptides determined by #5571 Anti-biotin (D5A7) Rabbit mAb (HRP Conjugate) detection using #7003 LumiGLO® Reagent and 20X Peroxide reagents.生物素化和未生物素化的肽段的相对荧光比较，所用y #5571 Anti-biotin (D5A7) Rabbit mAb (HRP Conjugate)，检测采用detection using #7003 LumiGLO ® Reagent 和 20X Peroxide reagents.

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货号：

5571S

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供应商现货

1ml

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