Blue Prestained Protein Marker Detection Pack

Product Usage Information

Important: Do Not Boil Protein Marker1. Thaw the protein ladder on ice.2. Gently vortex solution to ensure the mixture is homogeneous.3. Load the appropriate volume of the protein marker per lane, as specified below:Mini-Gel:0.75-1.0 mm thick: load 5 μl1.5 mm thick: load 10 μlLarge-Gel:0.75-1.0 mm thick: load 10 μl1.5 mm thick: load 20 μlPlease note, when using #59329 Blue Prestained Protein Marker, Broad Range (11-250 kDa) with #46387 Anti-Blue (2D2F11) Mouse mAb (HRP Conjugate), it is recommended to load between 0.5 μl-2.5 μl of #59329 to better visualize the separation of proteins while minimizing excess background due to an overabundance of protein.4. Unused ladder may be returned to -20ºC for long-term storage.Detection of Protein Ladders:a) Perform chemiluminescent detection according to the manufacturer's instructions except, at the stage of secondary antibody incubation with the blot, concurrently add the Anti-Blue (2D2F11) Mouse mAb (HRP Conjugate) at a dilution of 1:1000. Incubate for one house at room temperature with gentle agitation.b) Proceed as usual with the detection procedure.