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rhBMP-4 GMP, CF (50 ug)
rhBMP-4 GMP, CF (50 ug)
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rhBMP-4 GMP, CF (50 ug)

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品牌： R&D Systems
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    产品介绍
    产品信息

    Scientific Data

    Bioactivity View Larger

    GMP-grade Recombinant Human BMP-4 (Catalog # 314E-GMP) induces BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 3-21 ng/mL.

    Bioactivity View Larger

    Equivalent bioactivity of GMP (Catalog # 314E-GMP) and Animal-Free (AFL314E) grades of Recombinant Human BMP-4 as measured by its ability to induce BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells (orange and green respectively).

    SDS-PAGE View Larger

    2 μg/lane of GMP-grade Recombinant Human BMP-4 Protein (314E-GMP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 8-9 kDa and 20-22 kDa, respectively.

    Carrier Free

    What does CF mean?

    CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

    What formulation is right for me?

    In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

    314E-GMP

    FormulationLyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
    ReconstitutionReconstitute at 50-200 μg/mL in sterile 4 mM HCl.
    ShippingThe product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 3 months, ≤ -65 °C under sterile conditions after reconstitution.

    Recombinant Human BMP-4 GMP Protein, CF Summary

    Product Specifications

    Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
    Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
    Activity
    Measured by its ability to induce BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 3-21 ng/mL.
    Source
    E. coli-derived human BMP-4 protein
    Lys303-Arg408
    Produced in an animal-free laboratory. Manufactured and tested under cGMP guidelines
    Accession #
    P12644.1
    N-terminal Sequence
    Analysis
    Identity was confirmed by mass spectrometry
    Structure / Form
    Disulfide-linked homodimer
    Predicted Molecular Mass
    12 kDa
    SDS-PAGE
    8-9 kDa, reducing conditions
    背景
    别名
    BMP2B,BMP-2B,BMP2B1,BMP2BMCOPS6,BMP4,BMP-4,Bone morphogenetic protein 2B,bone morphogenetic protein 4,DVR4,MCOPS6,OFC11,ZYME,Bone Morphogenetic Protein 4
    背景

    Background: BMP-4

    Bone morphogenetic protein 4 (BMP-4) is a TGF-beta superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair (1-5). It is an integral part of many stem cell differentiation pathways, including lung tissue, (6), adipogenesis (7) and osteogenesis (8, 9). The human BMP-4 precursor contains a 273 amino acid (aa) propeptide and a 116 amino acid (aa) mature protein (10). Processing of the propeptide by furin or proprotein convertase 6 enables the formation of the mature disulfide-linked homodimeric BMP-4 and facilitates its secretion. Similar intracellular processes may lead to the formation and recreation of BMP4/BMP7 disulfide-linked heterodimer (11-13). Mature human and mouse BMP-4 share 98% aa sequence identity. Human BMP-4 shares 85% aa sequence identity with human BMP-2 and less than 50% with other human BMPs. In Xenopus, BMP-4 dimers provide ventralizing signals for existing mesoderm (14). BMP-4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPR-IA) and type II (primarily Activin RIIA or BMPR-II) receptors (15, 16). The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycans (17-19).

    References
    1. Zhang, P. et al. (2008) Blood 111:1933.
    2. Gambaro, K. et al. (2006) Cell Death Differ. 13:1075.
    3. Simic, P. and S. Vukicevic (2005) Cytokine Growth Factor Rev. 16:299.
    4. Sadlon, T.J. et al. (2004) Stem Cells 22:457.
    5. Frank, D.B. et al. (2005) Circ. Res. 97:496.
    6. Lee, J-H. et al. (2014) Cell. 156:440.
    7. Tang, QQ. et Lane MD. (2012) Annu Rev. Biochem. 81:715.
    8. Urist, MR. (1965). Science. 150:893.
    9. Bandyopadhyay, A. et al. (2006) Plos Genetics. 2:e216.
    10. Wozney, J. et al. (1988) Science 242:1528.
    11. Cui, Y. et al. (1998) EMBO J. 17:4735.
    12. Cui, Y. et al. (2001) Genes Dev. 15:2797.
    13. Aono, A. et al. (1995) Biochem. Biophys. Res. Commun. 210:670.
    14. Nishimatsu, S. and G.H. Thomsen (1998) Mech. Dev. 74:75.
    15. Chen, D. et al. (2004) Growth Factors 22:233.
    16. Lavery, K. et al. (2008) J. Biol. Chem. April 24 epub.
    17. Rosen, V. (2006) Ann. N.Y. Acad. Sci. 1068:19.
    18. Jones, C.M. and J.C. Smith (1998) Dev. Biol. 194:12.
    19. Takada, T. et al. (2003) J. Biol. Chem. 278:43229.
    Long Name
    Bone Morphogenetic Protein 4
    Entrez Gene IDs
    652 (Human); 12159 (Mouse); 25296 (Rat); 30612 (Zebrafish)
    Alternate Names
    BMP2B; BMP-2B; BMP2B1; BMP2BMCOPS6; BMP4; BMP-4; Bone morphogenetic protein 2B; bone morphogenetic protein 4; DVR4; MCOPS6; OFC11; ZYME
    研究领域
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    货号：
    314E-GMP-050
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    50ug
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