BrdU Cell Proliferation Chemiluminescent Assay Kit

Figure 1. C2C12 cells were seeded at varying density in serum free medium in a 96-well plate and incubated overnight. Serum was added to the plate at various concentrations and cells were incubated for 24 hr. Finally, 10 μM BrdU was added to the plate and cells were incubated for 4 hr.将C2C12细胞以各种浓度接种到96孔板中进行无血清培养基培养过夜。随后加入各种不同浓度的血清并将细胞培养24小时。最后，加入10 μM BrdU孵育4小时。

Figure 3. Jurkat cells were seeded at 5x104 cells/well in a 96-well plate and incubated overnight. Cells were then treated with various concentrations of doxorubicin for 2 hr. Finally, 10 μM BrdU was added to the plate and cells were incubated for 4 hr.Jurkat细胞在96孔板中以5x104 cells/well的密度铺板，培养过夜。加入不同浓度的阿霉素处理细胞2小时。最后，加入10 μM BrdU孵育4小时。

Figure 2. Treatment of MCF 10A cells with Human Epidermal Growth Factor (hEGF) #8916 increases cell proliferation as detected by the BrdU Cell Proliferation Chemiluminescent Assay Kit #5492. MCF 10A cells were seeded at 1x104 cells/well in a 96-well plate and incubated overnight. Cells were then starved in serum free medium overnight. hEGF was added to the plate and cells were incubated for 24 hr. Finally, 10 μM BrdU was added to the plate and cells were incubated for 4 hr.用人表皮生长因子（hEGF）#8916处理MCF10A细胞可以促进细胞增殖，该过程经BrdU细胞增殖化学发光检测试剂盒#5492检测证实。将MCF10A细胞在96孔板中以1x104 cells/well的密度铺板，培养过夜。过夜时使用无血清培养基饥饿处理。随后加入hEGF培养24小时。最后，加入10 μM BrdU孵育4小时。