BrdU Cell Proliferation Assay Kit

品牌： CST

下载产品说明书

用小程序，查商品更便捷

对比

咨询

参数

实验应用：

产品介绍

产品信息

产品详情

Cell Cycle / Checkpoint Control

简单描述

Assay Kit for studying BrdU in the research area.

组成成分

5-溴-2-脱氧尿苷水溶液0.06%，固定液（0.1%-0.5%氢氧化钠水溶液）16.6%，5-溴-2-脱氧尿苷抗体0.17%，抗小鼠免疫球蛋白G抗体、偶联HRP（辣根过氧化物酶）0.17%，检测抗体稀释液（磷酸盐缓冲液）16.6%,辣根过氧化物酶稀释液（三(羟甲基)氨基甲烷溶液)16.6%，TMB底物（三(羟甲基)氨基甲烷溶液)16.6%，终止液（顺丁烯二酸溶液）16.6%，洗涤缓冲液（0.5%5-氯-2-甲基-3(2H)异噻唑酮、2-甲基3(2H)异噻唑酮溶液）16.6%

应用

实验应用

目标/特异性

Specificity/Sensitivity

BrdU Cell Proliferation Assay kit detects BrdU incorporation into cellular DNA during cell proliferation. The BrdU-labeled DNA has to be denatured to be detected by the BrdU Mouse mAb used in this kit. This BrdU Mouse mAb does not cross react with endogenous DNA. Depending on the cell type and the incubation time applied in the assay, 0.2-2x104 cells/well are sufficient for most experimental setups. For the best result, a cell number titration (Figure 1) is recommended.

背景

Halogenated nucleotides such as the pyrimidine analog bromodeoxyuridine (BrdU) are useful for labeling nascent DNA in living cells and tissues. BrdU becomes incorporated into replicating DNA in place of thymidine and subsequent immunodetection of BrdU using specific monoclonal antibodies allows labeling of cells in S phase of the cell cycle. After pulse-labeling cells or tissues with bromodeoxyuridine, BrdU (Bu20a) Mouse mAb can be used to detect BrdU incorporated into single stranded DNA. Please see our detailed protocol for information regarding the labeling procedure and denaturation of double stranded DNA for various immunodetection applications (1-4). 1.Darzynkiewicz, Z. and Juan, G. (2001) Curr Protoc Cytom Chapter 7, Unit 7.7. 2.Leif, R.C. et al. (2004) Cytometry A 58, 45-52. 3.Staszkiewicz, J. et al. (2009) Biochem Biophys Res Commun 378, 539-44. 4.Rothaeusler, K. and Baumgarth, N. (2007) Curr Protoc Cytom Chapter 7, Unit7.31.

参考图片

Figure 1. C2C12 cells were seeded at varying density in serum free medium in a 96-well plate and incubated overnight. Serum was added to the plate at various concentrations and cells were incubated for 24 hr. Finally, 10 μM BrdU was added to the plate and cells were incubated for 4 hr.

Figure 2. Treatment of MCF 10A cells with Human Epidermal Growth Factor (hEGF) #8916 increases cell proliferation as detected by BrdU Cell Proliferation Assay Kit #6813. MCF 10A cells were seeded at 1x104 cells/well in a 96-well plate and incubated overnight. Cells were then starved in serum free medium overnight. hEGF was added to the plate and cells were incubated for 24 hr. Finally, 10 μM BrdU was added to the plate and cells were incubated for 4 hr.

Figure 3. Jurkat cells were seeded at 4x104 cells/well in a 96-well plate and incubated overnight. Cells were then treated with various concentrations of doxorubicin for 2 hr. Finally, 10 μM BrdU was added to the plate and cells were incubated for 4 hr.

声明：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。

货号：

6813S

一键复制

询价

1Kit

选择数量

当前规格1件起购

配送至

预计3-4周送达，快递: 免运费，若需干冰额外收费

询价大包装询价