Brilliant Stain Buffer
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Brilliant Stain Buffer

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品牌: BD Pharmingen
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    产品介绍
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    简单描述
    The BD Horizon™ Brilliant Stain Buffer is a buffer for the immunofluorescent staining of cells. Brilliant Stain Buffer is a solution that is added to mixtures of certain fluorescent reagents before staining cells. It was designed to complement multicolor flow cytometry experiments that utilize staining reagents conjugated with BD Horizon Brilliant fluorescent polymer dyes. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime BD Horizon Brilliant dyes are used. The BD Horizon Brilliant Stain Buffer is compatible with the use of other fluorescent staining reagents conjugated with traditional fluorochromes, such as fluorescein, phycoerythrin or Alexa Fluor® dyes.
    商品描述
    The BD Horizon™ Brilliant Stain Buffer is a buffer for the immunofluorescent staining of cells. Brilliant Stain Buffer is a solution that is added to mixtures of certain fluorescent reagents before staining cells. It was designed to complement multicolor flow cytometry experiments that utilize staining reagents conjugated with BD Horizon Brilliant fluorescent polymer dyes. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime BD Horizon Brilliant dyes are used. The BD Horizon Brilliant Stain Buffer is compatible with the use of other fluorescent staining reagents conjugated with traditional fluorochromes, such as fluorescein, phycoerythrin or Alexa Fluor® dyes.
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    Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
    文献
    文献
    Development References(7) 1. Acosta JR, Douagi I, Andersson DP, et al. Increased fat cell size: a major phenotype of subcutaneous white adipose tissue in non-obese individuals with type 2 diabetes.. Diabetologia. 2016; 59(3):560-70. (Methodology). 2. Andrés-Blasco I, Herrero-Cervera A, Vinué Á, et al. Hepatic lipase deficiency produces glucose intolerance, inflammation and hepatic steatosis.. J Endocrinol. 2015; 227(3):179-91. (Methodology). 3. Ingelman-Sundberg HM, Laestadius Å, Chrapkowska C, et al. Diverse effects on vaccine-specific serum IgG titres and memory B cells upon methotrexate and anti-TNF-α therapy in children with rheumatic diseases: A cross-sectional study.. Vaccine. 2016; 34(10):1304-11. (Methodology: Flow cytometry). 4. Leijten EF, van Kempen TS, Boes M, et al. Brief report: enrichment of activated group 3 innate lymphoid cells in psoriatic arthritis synovial fluid.. 2015; 67(10):2673-8. (Methodology: Flow cytometry). 5. McKay FC, Gatt PN, Fewings N, et al. The low EOMES/TBX21 molecular phenotype in multiple sclerosis reflects CD56+ cell dysregulation and is affected by immunomodulatory therapies.. Clin Immunol. 2016; 163:96-107. (Methodology: Flow cytometry). 6. O'Connor KS, Read SA, Wang M, et al. IFNL3/4 genotype is associated with altered immune cell populations in peripheral blood in chronic hepatitis C infection.. Genes Immun. 2016; 17(6):328-34. (Methodology: Flow cytometry). 7. Vinué Á, Andrés-Blasco I, Herrero-Cervera A, et al. Ink4/Arf locus restores glucose tolerance and insulin sensitivity by reducing hepatic steatosis and inflammation in mice with impaired IRS2-dependent signalling.. Biochim Biophys Acta. 2015; 1852(9):1729-42. (Methodology: Flow cytometry).

    参考图片

    Figure 1. BD Horizon Brilliant Stain Buffer should be used in multicolor immunofluorescent staining and flow cytometric analysis when using two or more BD Horizon Brilliant reagents. Whole human blood was stained with BD Horizon™ BV421 Mouse Anti-Human CD4 antibody and either BD Horizon™ BV605 Mouse Anti-Human CD8 antibody (Left Panels) or BD Horizon™ BV786 Mouse Anti-Human CD19 antibody (Right Panel). Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). Two-color flow cytometric dot plots show the correlated expression patterns of CD4 versus either CD8 or CD19 for gated events with the forward and side light-scatter characteristics of viable lymphocytes. Staining cells in the presence of BD Horizon™ Brilliant Stain Buffer (Cat. No. 563794/566349; Lower Panels) restores the major lymphocyte populations to their expected fluorescent staining patterns (dotted lines) when compared with cells stained without Brilliant Stain Buffer (Upper Panels). Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.

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    货号:
    566349
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