Purified Mouse C3a Protein (native)

C3a

Purified Mouse C3a protein is a native protein isolated from activated mouse serum. Anaphylatoxin C3a is a bioactive cleavage product released from plasma component C3 during complement activation and is involved in mediation of a variety of cellular immune responses, as well as being a potent pro-inflammatory agent. The release of this cleavage product is a reliable indicator of in vivo or in vitro complement activation.  Mouse C3a protein was isolated from activated mouse serum by affinity chromatography. This product can be reconstituted using 1 mL of Assay Diluent (Cat. No. 555213) or equivalent neutral buffer containing carrier protein, aliquoted into single-use aliquots and stored at -80°C.  Avoid multiple freeze-thaws of the reconstituted protein.  Each vial contains enough protein for ten 96-well plates.  Failure to add carrier protein or store at indicated temperatures may result in a loss of activity.  This product should not be diluted prior to long-term storage.

Purified Mouse C3a protein is a native protein isolated from activated mouse serum. Anaphylatoxin C3a is a bioactive cleavage product released from plasma component C3 during complement activation and is involved in mediation of a variety of cellular immune responses, as well as being a potent pro-inflammatory agent. The release of this cleavage product is a reliable indicator of in vivo or in vitro complement activation.  Mouse C3a protein was isolated from activated mouse serum by affinity chromatography. This product can be reconstituted using 1 mL of Assay Diluent (Cat. No. 555213) or equivalent neutral buffer containing carrier protein, aliquoted into single-use aliquots and stored at -80°C.  Avoid multiple freeze-thaws of the reconstituted protein.  Each vial contains enough protein for ten 96-well plates.  Failure to add carrier protein or store at indicated temperatures may result in a loss of activity.  This product should not be diluted prior to long-term storage.

ELISA (Routinely Tested)

Lyophilized in an aqueous buffered solution containing BSA and ProClin™ 150.

Development References(4) 1. Ember J.A, Jagels M.A., Hugli T.E. Characterization of complement anaphylatoxins and their biologic responses. In: Volenakis JE and Frank MM, ed. The human complement system in health and disease. New York: Marcel Dekker Inc; 1998:241-284. 2. Hugli T.E. Structure and function of C3a anaphylatoxin . Curr Top Microbiol Immunol. 1990; 153:181-208. (Biology). 3. Hugli T.E. Structure and function of the anaphylatoxins . Springer Semin Immunopathol. 1984; 7:193-219. (Biology). 4. Volanakis J.E. Overview of the complement system . In: Volenakis JE and Frank MM, ed. The human complement system in health and disease. New York: Marcel Dekker Inc; 1998:9-32.