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BD Pharmingen™ Calcein AM
BD Pharmingen™ Calcein AM
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BD Pharmingen™ Calcein AM

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品牌： BD Pharmingen
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    实验应用：
    Bioimaging, Flow cytometry (Tested During Development)
    Bioimaging, Flow cytometry (Tested During Development)
    产品介绍
    产品信息
    简单描述
    BD Pharmingen™ Calcein AM is used for labeling live cells that can be detected and further analyzed by flow cytometry or fluorescence imaging. The enhanced hydrophobicity of the acetomethoxy (AM) derivative of Calcein allows this dye to readily enter viable cells. Once inside, intracellular esterases cleave the AM groups off of the non-fluorescent BD Pharmingen™ Calcein AM, trapping fluorescent Calcein within the cell. Since dead cells lack esterase activity, only viable cells are labeled. Calcein AM is optimally excited at the 495 nm wavelength of light and emits maximally at 515 nm.
    商品描述
    BD Pharmingen™ Calcein AM is used for labeling live cells that can be detected and further analyzed by flow cytometry or fluorescence imaging. The enhanced hydrophobicity of the acetomethoxy (AM) derivative of Calcein allows this dye to readily enter viable cells. Once inside, intracellular esterases cleave the AM groups off of the non-fluorescent BD Pharmingen™ Calcein AM, trapping fluorescent Calcein within the cell. Since dead cells lack esterase activity, only viable cells are labeled. Calcein AM is optimally excited at the 495 nm wavelength of light and emits maximally at 515 nm.
    克隆号
    (RUO)
    应用
    实验应用
    Bioimaging, Flow cytometry (Tested During Development)
    目标/特异性
    Calcein Analogs
    文献
    文献
    研发参考(4) 1. Coder DM. Assessment of cell viability. Curr Protoc Cytom. 2013; Ch. 9(Unit 9.2):1-26. (Methodology). 2. Gatti R, Belletti S, Orlandini G, Bussolati O, Dall'Asta V, Gazzola GC. Comparison of annexin V and calcein-AM as early vital markers of apoptosis in adherent cells by confocal laser microscopy. J Histochem Cytochem. 1998; 46(8):895-900. (Biology). 3. Giordano G, Hong S, Faustman EM, Costa LG. Measurements of cell death in neuronal and glial cells. Methods Mol Biol. 2011; 758:171-178. (Biology). 4. Huitink GM, Poe DP, Diehl H. On the properties of Calcein Blue. Talanta. 1974; 21(12):1221-1229. (Biology).

    参考图片

    Flow cytometric analysis of BD Pharmingen™ Calcein AM fluorescence in Jurkat Cells. Cells from the human Jurkat (Acute T cell leukemia, ATCC TIB-152) cell line were treated with 0.025% DMSO (Left Panel) or 5 μM camptothecin (Right Panel) for 20 hours and then stained with 10 μM BD Pharmingen™ Calcein AM (Cat. No. 564061; solid line histograms) in serum-free buffer. Unstained cells are indicated by the dashed line histograms. Non-viable cells show a decrease in fluorescence intensity. Histograms were derived from gated events with the light scattering characteristics of Jurkat cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System. Calcein AM has also been tested on mouse (data not shown).

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    货号：
    564061
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