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Phospho-CaMKII (Thr286) (D21E4) Rabbit mAb

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Phospho-CaMKII (Thr286) (D21E4) Rabbit mAb

品牌： CST

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参数

分子量：

60, 50

反应种属：

Human,Mouse,Rat,

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产品介绍

产品信息

抗原名称

CaMKII

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr287 of human CamKII-β protein.

宿主

Rabbit

简单描述

Monoclonal Antibody for studying CaMK2-beta (Thr287) phosphate/CaMK2-delta (Thr287) phosphate/CaMK2-gamma (Thr287) phosphate/CaMK2-alpha (Thr286) phosphate. Cited in 221 publications. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-CaMKII (Thr286) (D21E4) Rabbit Monoclonal Antibody (CST #12716) is ready to ship.

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000

分子量

60, 50

应用

反应种属

Human,Mouse,Rat,

目标/特异性

Specificity/Sensitivity

Phospho-CaMKII (Thr286) (D21E4) Rabbit mAb recognizes endogenous levels of CamKII-α protein only when phosphorylated at Thr286. This antibody also recognizes endogenous levels of CamKII-β and CamKII-γ protein only when phosphorylated at Thr287.

Species Reactivity:

Human, Mouse, Rat

敏感性

Endogenous

背景

CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T cell receptor signaling (1,2). CaMKII has catalytic and regulatory domains. Ca2+/calmodulin binding to the CaMKII regulatory domain relieves autoinhibition and activates the kinase (3). The activated CaMKII further autophosphorylates at Thr286 to render the kinase constitutively active (3). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. PKA (protein kinase A) prevents phospho-CaMKII (Thr286) dephosphorylation through an inhibitory effect on PP1 (4). 1.Hughes, K. et al. (2001) J Biol Chem 276, 36008-13. 2.Barria, A. et al. (1997) Science 276, 2042-5. 3.Barkai, U. et al. (2000) Mol Endocrinol 14, 554-63. 4.Makhinson, M. et al. (1999) J Neurosci 19, 2500-10.

翻译后修饰

Phosphate

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

数据库链接

Entrez-Gene ID

816,817,818,815

UniProt ID

Q13554,Q13557,Q13555,Q9UQM7

参考图片

Western blot analysis of extracts from 293T cells, untransfected or transfected with specific CamKII proteins as indicated, using Phospho-CamKII (Thr286) (D21E4) Rabbit mAb. Antibody phosphospecificity was verified by preincubating the antibody in the absence of a peptide (-) or with either CamKII-β (Thr287) phosphopeptide (+) or CamKII-β (Thr287) non-phosphopeptide (+) prior to incubating the membrane.

Western blot分析未转染或转染了特异的CamKII蛋白的293T细胞提取物，使用的抗体是Phospho-CamKII (Thr286) (D21E4) Rabbit mAb 兔单抗。通过将抗体与对照(-)或CamKII-β (Thr287) phosphopeptide (+) 或 CamKII-β (Thr287) non-phosphopeptide (+)预孵育，再与膜孵育，以确定抗体的磷酸化特异性。

Western blot analysis of extracts from rat brain, mouse brain and human cerebellum using Phospho-CaMKII (Thr286) (D21E4) Rabbit mAb.

Western blot分析大鼠大脑，小鼠大脑和人小脑提取物，使用的抗体是Phospho-CaMKII (Thr286) (D21E4) Rabbit mAb 兔单抗。

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货号：

12716T

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20ul

100ul

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