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Caspase-3 (D3R6Y) Rabbit mAb

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Caspase-3 (D3R6Y) Rabbit mAb

品牌： CST

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参数

分子量：

35, 19, 17

反应种属：

Human,Mouse,Rat,Monkey,

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产品介绍

产品信息

抗原名称

Caspase-3

来源纯化

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the p20 subunit of human caspase-3 protein.

宿主

Rabbit

简单描述

Monoclonal Antibody for studying Casp3. Cited in 374 publications. Validated for WB, WB, IP. Available in 2 sizes. Highly specific and rigorously validated in-house, Caspase-3 (D3R6Y) Rabbit Monoclonal Antibody (CST #14220) is ready to ship.

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Simple Western™	1:10 - 1:50
Immunoprecipitation	1:100

分子量

35, 19, 17

内毒素水平

猪

研究领域

癌症,细胞生物学,纤维化,神经科学,

应用

反应种属

Human,Mouse,Rat,Monkey,

预测反应种属

Pig

目标/特异性

Specificity/Sensitivity

Caspase-3 (D3R6Y) Rabbit mAb recognizes endogenous levels of total caspase-3 protein. This antibody detects full-length caspase-3 as well as the large subunit (p20) of caspase-3 resulting from cleavage during apoptosis.

Species Reactivity:

Human, Mouse, Rat, Monkey

敏感性

Endogenous

背景

Caspase-3 (CPP-32, Apopain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2). 1.Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4. 2.Nicholson, D.W. et al. (1995) Nature 376, 37-43.

研究领域

癌症,细胞生物学,纤维化,神经科学,

翻译后修饰

Unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

数据库链接

Entrez-Gene ID

836

UniProt ID

P42574

参考图片

Western blot analysis of various cell lines, untreated (-) or treated with Staurosporine #9953 (1 μM; 3 hr) or with Etoposide #2200 (25 μM, overnight), using Caspase-3 (D3R6Y) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). MCF7 cells are negative for caspase-3 expression.

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货号：

14220T

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