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Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb
Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb
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Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb

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品牌： CST
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    分子量：
    17, 19
    17, 19
    反应种属：
    Human,Mouse,Rat,Monkey,
    Human,Mouse,Rat,Monkey,
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    产品介绍
    产品信息
    抗原名称
    Cleaved Caspase-3
    来源纯化
    使用与人 caspase-3 Asp175 周围的氨基末端残基相对应的合成肽对动物进行免疫接种来产生单克隆抗体。
    宿主
    Rabbit
    简单描述
    Monoclonal Antibody for studying Casp3 (Asp175) cleaved. Cited in 5550 publications. Validated for WB, WB, IP, IHC, IF, F. Available in 3 sizes. Highly specific and rigorously validated in-house, Cleaved Caspase-3 (Asp175) (5A1E) Rabbit Monoclonal Antibody (CST #9664) is ready to ship.
    商品描述

    Product Usage Information

    ApplicationDilution
    Western Blotting1:1000
    Simple Western™1:10 - 1:50
    Immunoprecipitation1:50
    Immunohistochemistry (Paraffin)1:2000
    Immunofluorescence (Immunocytochemistry)1:400 - 1:1600
    Flow Cytometry (Fixed/Permeabilized)1:6400
    分子量
    17, 19
    内毒素水平
    牛 , 犬 , 猪
    研究领域
    癌症,细胞生物学,纤维化,神经科学,
    应用
    反应种属
    Human,Mouse,Rat,Monkey,
    预测反应种属
    Bovine,Dog,Pig,
    目标/特异性

    Specificity/Sensitivity

    Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 可检测 Asp175 附近裂解活化产生的内源水平的活化 caspase-3 中大片段 (17/19 kDa) 。该抗体无法检测全长 caspase-3 或其他剪切的半胱天冬酶。通过对固定冷冻组织中健康细胞（如胰腺 α 细胞）的特定亚型进行免疫荧光分析可能会观察到非特异性标记。在人和猴样本中可能会观察到细胞浆背景。

    物种反应性：

    人, 小鼠, 大鼠, 猴

    敏感性
    Endogenous
    背景
    背景
    Caspase-3（CPP-32、Apopain、Yama、SCA-1）是细胞凋亡的关键执行者，因为它部分或完全负责许多关键蛋白的蛋白水解剪切，例如核酶聚（ADP-核糖）聚合酶 (PARP) (1)。caspase-3 的激活需要经过其未活化的酶原形式转变为活化的 p17 和 p12 片段的蛋白水解过程。caspase-3 的剪切需要位于 P1 位点的天门冬氨酸残基 (2)。 1.Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4. 2.Nicholson, D.W. et al. (1995) Nature 376, 37-43.
    研究领域
    癌症,细胞生物学,纤维化,神经科学,
    翻译后修饰
    Cleaved
    制备和贮存
    保存方式
    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #94530.
    数据库链接
    Entrez-Gene ID
    836
    UniProt ID
    P42574

    参考图片

    Immunoprecipitation of extracts from Jurkat cells, untreated or etoposide-treated (25uM, 5hrs), using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb. Western blot was performed using the same antibody.

    免疫沉淀分析Jurkat 细胞的提取物，未处理或 etoposide （依托泊苷）(25uM, 5hrs)处理，采用Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 兔单抗，然后用同样抗体进行western blot 分析。

    Immunohistochemical staining of paraffin-embedded mouse embryo, showing cytoplasmic localization in apoptotic cells, using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb.

    免疫组化分析石蜡包埋小鼠胚胎，显示凋亡细胞中裂解的caspase-3定位胞浆，抗体采用 Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 兔单抗。

    Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with etoposide #2200 (green), using Cleaved Caspase-3(Asp175) (5A1E) Rabbit mAb compared to a nonspecific negative control antibody (red).

    Immunohistochemical analysis using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb on SignalSlide (TM) Cleaved Caspase-3 IHC Controls #8104 (paraffin-embedded Jurkat cell, untreated (left) or etoposide-treated (right)).

    免疫组化分析，用 SignalSlide (TM) Cleaved Caspase-3 IHC Controls #8104（石蜡包埋的Jurkat 细胞，未处理（左）或 etoposide 处理（右）），采用 Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 兔单抗。

    Western blot analysis of extracts from C6 (rat), NIH/3T3 (mouse), and Jurkat (human) cells, untreated or treated with staurosporine (1uM, 3hrs) or etoposide (25uM, 5hrs) as indicated, using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb.

    Western blot 分析未处理或 staurosporine （星胞菌素） (1uM, 3hrs) 或 etoposide （依托泊苷）(25uM, 5hrs)处理的C6 (大鼠源)、NIH/3T3 (小鼠源)和Jurkat (人源) 细胞。抗体采用Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 兔单抗。

    Immunohistochemical analysis of paraffin-embedded mouse embryo, using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb in the presence of control peptide (left) or Cleaved Caspase-3 (Asp175) Blocking Peptide (#1050) (right).

    免疫组化分析石蜡包埋的小鼠胚胎，抗体采用Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 兔单抗，并用对照肽段（左图）或 Cleaved Caspase-3 (Asp175) 封闭肽段 (#1050) 对照(右图)。

    Confocal immunofluorescent images of HT-29 cells, untreated (left) or Staurosporine treated (right) labeled with Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb#9953 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

    共聚焦免疫荧光分析未处理（左）或 Staurosporine (星胞菌素)处理（右）的HT-29 细胞，抗体采用 Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 兔单抗#9953（绿色）。微丝用Alexa Fluor® 555 phalloidin（红色）标记。蓝色伪彩=DRAQ5® #4084 (DNA 荧光染料)。

    Immunohistochemical analysis of frozen H1650 xenograft, using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb.

    免疫组化分析 H1650 xenograft 冰冻切片，抗体采用Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 兔单抗。

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    货号：
    9664S
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