BD Horizon™ RB705 Mouse Anti-Human CD3

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BD Horizon™ RB705 Mouse Anti-Human CD3

品牌： BD Pharmingen

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反应种属：

Human (QC Testing)

实验应用：

Flow cytometry (Routinely Tested)

产品介绍

产品信息

耦联标记

RB705

免疫原

Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor

简单描述

The UCHT1 monoclonal antibody specifically binds to the human CD3ε-chain, a 20-kDa subunit of the CD3/T cell antigen receptor complex. CD3ε is expressed on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. Studies from the HLDA Workshop show that this antibody is mitogenic for CD3ε-positive cells when used in conjunction with costimulatory agents such as pokeweed mitogen or anti-CD28 antibody. CD3 plays a central role in signal transduction during antigen recognition. The UCHT1 antibody stains both surface and intracellular CD3ε unlike the other CD3 clone, HIT3a, that stains only extracellular CD3ε.

商品描述

UCHT1 The UCHT1 monoclonal antibody specifically binds to the human CD3ε-chain, a 20-kDa subunit of the CD3/T cell antigen receptor complex. CD3ε is expressed on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. Studies from the HLDA Workshop show that this antibody is mitogenic for CD3ε-positive cells when used in conjunction with costimulatory agents such as pokeweed mitogen or anti-CD28 antibody. CD3 plays a central role in signal transduction during antigen recognition. The UCHT1 antibody stains both surface and intracellular CD3ε unlike the other CD3 clone, HIT3a, that stains only extracellular CD3ε.

同种型

Mouse BALB/c IgG1, κ

克隆号

克隆 UCHT1 (also known as UCHT-1; UCHT 1) (RUO)

产品详情

RB705

The BD Horizon RealBlue™ 705 (RB705) Dye is part of the BD® family of blue dyes. It is a tandem fluorochrome with an excitation maximum (Ex Max) at 498-nm and an emission maximum (Em Max) at 707-nm as measured using an antibody-dye conjugate. Driven by BD® innovation, RB705 can be used on both spectral and conventional cytometers and is designed to be excited by the Blue laser (488-nm) with minimal excitation by the 561-nm Yellow-Green laser. For conventional instruments equipped with a Blue laser (488-nm), RB705 can be used as an alternative to PerCP-Cy5.5 or BB700 and we recommend using an optical filter centered near 710-nm (e.g., a 695/40 or 710/50-nm bandpass filter). For spectral instruments equipped with a Blue laser (488-nm), it can be used in conjunction with PerCP-Cy5.5. RB705 is on average brighter than PerCP-Cy5.5 and BB700, and has minimal spillover into Yellow-Green detectors.

格式:RB705

激发源:Blue 488 nm

最多激发源:498 nm

最多发射源:707 nm

应用

实验应用

Flow cytometry (Routinely Tested)

推荐用量

5 µl/test

反应种属

Human (QC Testing)

目标/特异性

CD3

背景

别名

CD3e; CD3E; T3E; TCRE; T-cell surface antigen T3/Leu-4 epsilon

制备和贮存

存储溶液

Aqueous buffered solution containing ≤0.09% sodium azide.

数据库链接

Entrez-Gene ID

916

参考图片

Multiparameter flow cytometric analysis of CD3 expression on Human peripheral blood leukocyte populations. Human whole blood was stained with either BD Horizon™ RB705 Mouse IgG1, κ Isotype Control (Cat. No. 570261; Left Plot) or BD Horizon™ RB705 Mouse Anti-Human CD3 antibody (Cat. No. 570237/570238; Right Plot). The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The bivariate pseudocolor density plot showing the correlated expression of CD3 (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony™ A5 SE Flow Cytometer System and FlowJo™ software.

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货号：

570237

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