Product Details

The anti-mouse CD3 F(ab’)2 fragment is the antigen binding F(ab’)2 fragments of the 145-2C11 monoclonal antibody only. The majority of the Fc fragment has been removed via pepsin digestion. The 145-2C11 antibody F(ab’)2 fragment reacts with mouse CD3ε, a 20 kDa transmembrane cell-surface protein that belongs to the immunoglobulin superfamily. CD3ε is one of five polypeptide chains that combine to form the TCR complex. CD3ε is expressed on T lymphocytes, NK-T cells, and to varying degrees on developing thymocytes. CD3 plays roles in TCR signaling, T lymphocyte activation, and antigen recognition. The complete 145-2C11 antibody is commonly used to stimulate T cells in vitro however, when used in vivo activation of resting T cells results in cytokine release and toxicity caused by Ab-mediated cross-linking of T cells and Fcγ receptor-bearing cells. To avoid these complications non-Fc receptor binding F(ab’)2 fragments of the 145-2C11 antibody are commonly used. This non-FcR-binding anti-CD3 induces apoptosis of Ag-activated T cells in vivo by allowing durable expression of the TCR and sustained signaling. Foxp3+ Tregs have been shown to be resistant to CD3 antibody-mediated depletion.

Specifications

Isotype	Armenian Hamster IgG1
Recommended Isotype Control(s)	InVivoMAb hamster IgG f(ab')2 fragments
Recommended Dilution Buffer	InVivoPure pH 7.0 Dilution Buffer
Immunogen	Mouse BM10-37 cytotoxic T cells
Reported Applications	in vivo T cell depletion
Formulation	PBS, pH 7.0 Contains no stabilizers or preservatives
Endotoxin	<2EU/mg (<0.002EU/μg) Determined by LAL gel clotting assay
Purity	>95% Determined by SDS-PAGE
Sterility	0.2 μM filtered
Production	Pepsin Digest
Purification	Protein A
RRID	AB_2687679
Storage	The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

组成成分

跨膜细胞表面蛋白抗体，磷酸盐缓冲液

背景

研究领域

Immunology

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货号：

BE0001-1FAB-5MG

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