BD IMag™ Human Naive CD4 T Cell Enrichment Set - DM

品牌： BD Pharmingen

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实验应用：

Cell separation (Routinely Tested)

产品介绍

产品信息

简单描述

The BD IMag™ Human Naïve CD4 + T Cell Enrichment Set - DM is used for the negative selection of untouched naïve CD4 + T cells from peripheral blood. The Human Naïve CD4 + T Cell Enrichment Cocktail contains biotinylated monoclonal antibodies that recognize antigens expressed on memory T cells, CD8 + T cells, B cells, NK cells, γ/δ T cells, monocytes, DCs, granulocytes, platelets, and erythroid cells. The BD IMag™ Streptavidin Particles Plus - DM are magnetic nanoparticles that have streptavidin covalently conjugated to their surfaces. With these two components, this enrichment set avoids the inadvertent activation of the enriched naïve helper T cells by using reagents that do not directly bind to those T cells. This product has been optimized for use with the BD IMag™ Cell Separation Magnet (Cat. No. 552311), and contains sufficient reagents to label 1× 10E9 peripheral blood mononuclear cells (PBMC). The Human Naïve CD4 + T Cell Enrichment Cocktail comprises the following monoclonal antibodies: Biotin Mouse Anti-human CD8, clone SK1 Biotin Mouse Anti-human CD11b, clone ICRF44 Biotin Mouse Anti-human CD16, clone 3G8 Biotin Mouse Anti-human CD19, clone HIB19 Biotin Mouse Anti-human CD36, clone CB38 Biotin Mouse Anti-human CD41a, clone HIP8 Biotin Mouse Anti-human CD45RO, clone UCHL1 Biotin Mouse Anti-human CD56, clone B159 Biotin Mouse Anti-human CD123, clone 9F5 Biotin Mouse Anti-human γδ-TCR, clone B1 Biotin Mouse Anti-human Glycophorin A, clone GA-R2

克隆号

(RUO)

BD化合物表

描述
EntrezGene ID
Human Naive CD4 T Cell Enrichment Cocktail
N/A
Streptavidin Particles Plus – DM
N/A

应用

实验应用

Cell separation (Routinely Tested)

目标/特异性

CD4

参考图片

Enrichment of naïve CD4+ T cells from human peripheral blood. PBMC were labeled with the BD IMag™ Human Naïve CD4+ T cell Enrichment Set - DM (Cat. No. 558521) and separated on the BD IMag™ Cell Separation Magnet (Cat. No. 552311) according to the accompanying protocol. In panels A and B, cells were stained with a cocktail consisting of PE Anti-Human CD8 (Cat. No. 555367), CD11b (Cat. No. 555388), CD16 (Cat. No. 555407), CD19 (Cat. No. 555413), CD36 (Cat. No. 555455), CD45RO (Cat. No. 555493), CD56 (Cat. No. 555516), CD123 (Cat. No. 555644), CD235a (Cat. No. 555570), and γδ TCR (Cat No. 555717) to detect non-naïve CD4+ cells, and an APC Human CD45RA (Cat. No. 550855) to detect naïve cells. For panels C and D, cells were stained with APC Mouse Anti-Human CD4 antibody (Cat. No. 555349) and FITC Mouse Anti-Human CD45RA antibody (Cat. No. 555488) to show the percentage of naïve CD4+ T cells. Dead cells were excluded by staining with 7-Amino-actinomycin D (7-AAD) (Cat. No. 559925). Refer to the Enrichment Flow Chart to identify the cell populations in this figure. The percentage of naïve CD4+ T cells is indicated in each panel. Panels A and C show unseparated PBMCs while panels B and D show the twice-enriched fraction after three 6-minute magnetic separations with an additional 3-10 minute separation. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

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货号：

558521

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