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PE Mouse Anti-Rat CD80(3H5)

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PE Mouse Anti-Rat CD80(3H5)

品牌： BD Pharmingen

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反应种属：

Rat (QC Testing)

来源宿主：

Mouse BALB/c IgG1, κ

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产品介绍

产品信息

耦联标记

抗原名称

CD80 (B7-1)

宿主

Mouse BALB/c IgG1, κ

免疫原

Rat HTLV-1–transformed T-cell line Lewis-S1

简单描述

The 3H5 antibody specifically recognizes CD80 (B7-1), a member of the Ig superfamily of transmembrane proteins. CD80, a ligand for CD28 and CD152 (CTLA-4), is one of the accessory molecules that plays an important role in T cell-B cell costimulatory interactions. CD80 is predominantly expressed on antigen-presenting cells. It can be induced on splenic B cells by in vitro polyclonal activation such as LPS treatment. 3H5 mAb is reported to block the costimulatory function of rat CD80 and to immunoprecipitate CD80 from Lewis-S1 cell lysates.

商品描述

同种型

Mouse BALB/c IgG1, κ

克隆号

3H5

浓度

0.2 mg/ml

产品详情

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

格式:PE

激发源:Yellow-Green 488 nm, 532 nm, 561 nm

最多激发源:496 nm, 566 nm

最多发射源:576 nm

应用

实验应用

Flow cytometry (Routinely Tested)

反应种属

Rat (QC Testing)

背景

别名

B7-1; Cd80

制备和贮存

存储溶液

Aqueous buffered solution containing ≤0.09% sodium azide.

保存方式

Aqueous buffered solution containing ≤0.09% sodium azide.

文献

研发参考(3) 1. Bluestone JA. New perspectives of CD28-B7-mediated T cell costimulation. Immunity. 1995; 2(6):555-559. (Biology). 2. Damoiseaux JG, Yagita H, Okumura K, van Breda Vriesman PJ. Costimulatory molecules CD80 and CD86 in the rat; tissue distribution and expression by antigen-presenting cells. J Leukoc Biol. 1998; 64(6):803-809. (Biology). 3. Maeda K, Sato T, Azuma M, Yagita H, Okumura K. Characterization of rat CD80 and CD86 by molecular cloning and mAb. Int Immunol. 1997; 9(7):993-1000. (Immunogen).

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货号：

555014

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供应商现货

200ug

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