BD IMag™ Human CD8 T Lymphocyte Enrichment Set-DM

Enrichment of CD8 T lymphocytes from human blood. PBMC were labeled with the BD IMag™ Human CD8 T Lymphocyte Enrichment Set - DM and separated on the BD IMag™ Cell Separation Magnet (Cat. No. 552311) according to the accompanying protocol. Cells were stained with APC Mouse Anti-Human CD8 (Cat. No. 555369) to detect CD8 T lymphocytes and a mixture of PE Mouse Anti-Human CD4 (Cat. No. 555347), CD11b/ Mac-1  (Cat. No. 555388), CD16 (Cat. No. 555407), CD19 (Cat. No. 555413), CD36 (Cat. No. 555455), CD56 (Cat. No. 555516), CD123 (Cat. No. 554529), CD235a (Cat. No. 555570), and γδ TCR (Cat. No. 555717) monoclonal antibodies to detect non-CD8 T leukocytes and erythrocytes.  Dead cells were excluded by staining with Propidium Iodide Staining Solution (Cat. No. 556463). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system. Refer to the Enrichment Flow Chart to identify the cell populations represented in this figure. The percentage of CD8 T cells is indicated in the lower-right corner of each panel. Far left panel shows unseparated PBMC. Middle left panel shows the combined enriched fraction after three 6-minute magnetic separations. Middle right panel shows the twice-enriched fraction after an additional 6-minute separation of the cells shown in middle left panel. This additional separation step can result in 5-10% increased purity with less than a 5% decrease in recovery. Far right panel shows the positive fraction.