BD IMag™ Mouse CD8 T Lymphocyte Enrichment Set - DM

Enrichment of CD8 T lymphocytes from mouse spleen. BALB/c splenocytes were labeled with the BD IMag Mouse CD8 T Lymphocyte Enrichment Set - DM (Cat. No. 558471) and separated on the BD IMag™ Cell Separation Magnet(Cat. No. 552311) according to the accompanying protocol. To demonstrate the efficiency of the enrichment, cells were stained with FITC-conjugated anti-mouse CD8a mAb 53-6.7 (Cat. No. 553030/553031) and APC-conjugated anti-mouse CD3e mAb 145-2C11 (Cat. No. 553066) to detect CD8 T lymphocytes.  Dead cells were excluded by staining with Propidium Iodide (Cat. No. 556463). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system. Please refer to the Enrichment Flow Chart to identify the cell populations represented in this figure. The percentage of CD8 T cells is indicated in the upper-right corner of each panel. Far left panel shows unseparated splenocytes. Middle left panel shows the combined fraction after three 6-minute magnetic separations. Middle right panel shows the twice-enriched fraction after an additional 6-minute separation of the cells shown in middle left panel. This additional incubation can result in up to 5% increased purity with up to a 5% decrease in recovery. Far right panel shows the positive fraction.