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rfeCD8a, CF (50 UG)
rfeCD8a, CF (50 UG)

rfeCD8a, CF (50 UG)

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品牌： R&D Systems
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    Carrier Free

    What does CF mean?

    CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

    What formulation is right for me?

    In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

    9649-CD

    FormulationLyophilized from a 0.2 μm filtered solution in PBS.
    ReconstitutionReconstitute at 250 μg/mL in PBS.
    ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

    Recombinant Feline CD8 alpha Protein, CF Summary

    Product Specifications

    Purity
    >85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
    Endotoxin Level
    <1.0 EU per 1 μg of the protein by the LAL method.
    Activity
    Bioassay data are not available.
    Source
    Mouse myeloma cell line, NS0-derived feline CD8 protein
    Ala22-Tyr188, with a C-terminal 6-His tag
    Accession #
    P41688
    N-terminal Sequence
    Analysis
    Ala22
    Predicted Molecular Mass
    19 kDa
    SDS-PAGE
    28-31 kDa, reducing conditions
    背景
    别名
    CD_antigen: CD8a,CD8 antigen, alpha polypeptide (p32),CD8,CD8a molecule,CD8A,Leu2 T-lymphocyte antigen,LEU2,MAL,OKT8 T-cell antigen,p32,T cell co-receptor,T8 T-cell antigen,T-cell antigen Leu2,T-cell surface glycoprotein CD8 alpha chain,T-lymphocyte differentiation antigen T8/Leu-2
    背景

    Background: CD8

    CD8 is a dimeric complex made up of two Ig superfamily members (1). The CD8 alpha chain is a type I transmembrane glycoprotein that can dimerize with either itself forming CD8 alpha alpha, or with a 30-35 kDa CD8 beta chain, forming CD8 alpha beta (2). CD8 alpha contains one V-type Ig-like domain in its extracellular region that binds to class I MHC molecules. CD8 alpha beta is a TCR co-receptor, while CD8 alpha alpha promotes T cell survival and differentiation (1, 3). The mature feline CD alpha consists of a 167 amino acid (aa) extracellular domain (ECD), and runs at ~30 kDa on reduced SDS-PAGE. Within the ECD, feline CD8 alpha shares 58%, 47%, 51%, and 69% amino acid sequence identity with human, mouse, porcine, and canine CD8 alpha, respectively.

    References
    1. Laugel, B. et al. (2011) J. Leukoc. Biol. 90:1089.
    2. Snow, P.M. and C. Terhorst (1983) J. Biol. Chem. 258:14675.
    3. Cole, D.K. et al. (2012) Immunology 137:139.
    Entrez Gene IDs
    925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)
    Alternate Names
    CD_antigen: CD8a; CD8 antigen, alpha polypeptide (p32); CD8; CD8a molecule; CD8A; Leu2 T-lymphocyte antigen; LEU2; MAL; OKT8 T-cell antigen; p32; T cell co-receptor; T8 T-cell antigen; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2
    研究领域
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    货号：
    9649-CD-050
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    询价
    50UG
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