
产品介绍
产品信息
抗原名称
CD8alpha

来源纯化
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp42 of mouse CD8α protein.

宿主
Rabbit

简单描述
CD8α antibody for detecting a subset of activated peripheral CD4+ T cells. Validated for WB, IHC, IHC. Available in 2 sizes. Click here to learn more.

商品描述
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
IHC Leica Bond | 1:200 - 1:800 |
Immunohistochemistry (Paraffin) | 1:200 - 1:800 |

分子量
35-42

研究领域
癌症,纤维化,免疫学和肿瘤学,神经科学,

应用
反应种属
Mouse

目标/特异性
Specificity/Sensitivity
CD8α (D4W2Z) XP® Rabbit mAb recognizes endogenous levels of total CD8α protein.
Species Reactivity:
Mouse

敏感性
Endogenous

背景
背景
Cluster of Differentiation 8 (CD8) is a disulphide-linked heterodimer consisting of the unrelated α and β subunits. Each subunit is a glycoprotein composed of a single extracellular Ig-like domain, a polypeptide linker, a transmembrane part and a short cytoplasmic tail. On T cells, CD8 is the coreceptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen–Major Histocompatibility Complex (MHC). Specifically, the Ig-like domain of CD8α interacts with the α3-domain of the MHC class I molecule. CD8 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and the α chain recruits the tyrosine kinase Lck, which is essential for T cell activation (1).
1.Zamoyska, R. (1994) Immunity 1, 243-46.

研究领域
癌症,纤维化,免疫学和肿瘤学,神经科学,
翻译后修饰
Unmodified

制备和贮存
保存方式
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #60168.
数据库链接
Entrez-Gene ID
12525

UniProt ID
P01731

参考图片
Western blot analysis of extracts from mouse CD8+ T cells, mouse spleen, EL4 cells, and Raw264.7 cells using CD8α (D4W2Z) XP® Rabbit mAb (Mouse Specific) (upper), and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunohistochemical analysis of paraffin-embedded mouse spleen using CD8α (D4W2Z) XP® Rabbit mAb (Mouse Specific).
Immunohistochemical analysis of paraffin-embedded mouse small intestine using CD8α (D4W2Z) XP® Rabbit mAb (Mouse Specific).
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