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Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunohistochemistry (Paraffin) | 1:200 - 1:800 |
Immunofluorescence (Immunocytochemistry) | 1:400 - 1:1600 |
Flow Cytometry (Fixed/Permeabilized) | 1:200 - 1:800 |






Specificity/Sensitivity
物种反应性:
人, 小鼠, 大鼠, 仓鼠 , 猴, 非洲爪蟾蜍, 斑马鱼 , 牛 , 猪






参考图片
Confocal immunofluorescent analysis of A-204 cells using HSP60 (D6F1) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
采用共聚焦免疫荧光分析A-204细胞,使用的抗体为HSP60 (D6F1) XP® Rabbit mAb兔单抗 (绿色)。肌动蛋白微丝使用DY-554 phalloidin进行标记(红色)。蓝色伪彩= DRAQ5® #4084 (荧光DNA染料)。
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using HSP60 (D6F1) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
免疫组化分别检测在对照多肽(左)或抗原特异性多肽(右)存在的情况下石蜡包埋的人卵巢癌,所使用的抗体为 HSP60 (D6F1) XP® Rabbit mAb兔单抗。
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using HSP60 (D6F1) XP® Rabbit mAb.
免疫组化检测石蜡包埋的人乳腺癌,所使用的抗体为 HSP60 (D6F1) XP® Rabbit mAb兔单抗。
Western blot analysis of extracts from various cell lines using HSP60 (D6F1) XP® Rabbit mAb.
Western blot方法检测多种细胞系的提取物,使用的抗体为HSP60 (D6F1) XP® Rabbit mAb兔单抗。
Flow cytometric analysis of HeLa cells using HSP60 (D6F1) XP® Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
流式细胞术检测HeLa细胞活性,使用的抗体为HSP60 (D6F1) XP® Rabbit mAb兔单抗 (蓝色)与使用浓度匹配的Rabbit (DA1E) mAb IgG XP® Isotype Control #3900的相比较(红色)。Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412作为二抗使用。
Immunohistochemical analysis of paraffin-embedded mouse lung using HSP60 (D6F1) XP® Rabbit mAb.
免疫组化检测石蜡包埋的小鼠肺组织,所使用的抗体为 HSP60 (D6F1) XP® Rabbit mAb兔单抗。